مقایسۀ اثر فشار ا کسایشی ناشی از ده هفته شنای متوسط و طولانیمدت بر رگزایی بطن چپ موشهای صحرایی نر

اردکانی زاده, ملیحه; وصالی اکبرپور, لیلا

doi:10.52547/joeppa.14.1.119

مقایسۀ اثر فشار ا کسایشی ناشی از ده هفته شنای متوسط و طولانیمدت بر رگزایی بطن چپ موشهای صحرایی نر

نوع مقاله : علمی - پژوهشی

نویسندگان

¹ گروه علوم ورزشی، دانشکدۀ علوم انسانی، دانشگاه دامغان، دامغان، ایران

² دانشکدۀ علوم ورزشی، دانشگاه بوعلی سینا، همدان، ایران

10.52547/joeppa.14.1.119

چکیده

هدف: در مطالعات پیشین، بین رگ‌زایی و فشار اکسایشی رابطه‌ای عنوان شده است؛ بنابراین، پژوهش حاضر با هدف مقایسۀ اثر دو پروتکل متوسط و طولانی‌مدت بر رگ‌زایی بطن چپ موش‌های صحرایی نر انجام گرفت.
روش‌ها: تعداد 18 سر موش نر صحرایی (20±210 گرم) به‌صورت تصادفی به سه گروه همتای کنترل، شنای متوسط و طولانی‌مدت تقسیم شدند. گروه‌های تمرینی به مدت 10 هفته و 5 روز در هفته در آب 32 درجۀ سانتی‌گراد شنا کردند. در هر جلسه گروه متوسط یک ساعت و گروه طولانی سه ساعت شنا کردند. یک روز پس از پایان تمرین، بافت بطن چپ قلب جدا شد و برای سنجش بیان ژن‌های عامل ‌رشد اندوتلیال‌ عروقی B، عامل ‌رشد فیبروبلاست B، آنژیوپویتین 1و2 و ماتریکس‌ متالوپروتئیناز 2 از روش real-time PCR استفاده شد. تفاوت‌ها با روش آنوا یکطرفه، و مقایسۀ گروه‌ها از طریق آزمون تعقیبی توکی در سطح معناداری 05/0p< انجام گرفت.
نتایج: مقادیر بیان ژن‌های عامل‌ رشد اندوتلیال ‌عروقی B، آنژیوپویتین 1و2، و ماتریکس ‌متالوپروتئیناز 2 در گروه طولانی‌مدت در مقایسه با هر دو گروه کنترل و متوسط، و در گروه متوسط نیز در مقایسه با گروه کنترل افزایش معناداری یافت، اما بیان ژن عامل ‌رشد فیبروبلاست B فقط در گروه متوسط در مقایسه با گروه کنترل افزایش معناداری داشت (001/0=P).
نتیجه‌گیری: با توجه به افزایش معنادار نیتریک ‌اکسید، می‌توان رخداد فرایند رگ‌زایی در گروه طولانی‌مدت را به فشار برشی، و در گروه متوسط به افزایش معنادار مالون‌ دی‌آلدئید، به فشار اکسایشی نسبت داد.

کلیدواژه‌ها

عنوان مقاله [English]

Comparison of the effects of 10 weeks mid and long-term swimming induced oxidative stress on left ventricular angiogenesis in male rats

نویسندگان [English]

Maliheh Ardakani-zadeh ¹
Leila Vesali-akbarpour ²

¹ Department of Sports Science, Faculty of Humanities, Damghan University, Damghan, Iran

² Faculty of Sports Sciences, Bu Ali Sina University, Hamadan, Iran

چکیده [English]

Purpose: In previous studies, there was a relationship between angiogenesis and oxidative stress thus the aim of this study was to compare the effect of two protocols of mid and long-term on left ventricular angiogenesis in male rats.
Methods: The 18 male Wistar rats of 210 ± 20g, were randomly assigned in to three equal groups: control, mid and long-term group. Training groups swam in 32^֯ water five days per week for 10 weeks. The mid-term group swam for one hour and the long-term group for three hours per session. One day after the end of the protocol, left ventricle of the heart was removed. Real-time PCR method was used to measure the genes expression of Vascular-endothelial-growth-factor-B, Fibroblast-Growth-Factor-B, Angiopoiteine1&2. Differences were determined using by ANOVA One-Way method and the groups were compared by Tukey follow-up test at the significant level of P < 0.05.
Results: The genes expression levels of Vascular-endothelial-growth factor-B, Angiopoiteine1&2 and Matrix-metalloproteinases-2 were significantly increased in the long-term group in compared to the both the control and mid-term groups, and in the mid-term group in compared to the control group, but the gene expression of Fibroblast-Growth-Factor-B had a significant increase only in the mid-term group in compared to the control group (P = 0.001).
Conclusion: Due to the significantly increase of nitric-oxide, the angiogenesis process in the long-term group can be related to shear stress, and in the mid-term group, due to a significant increase in Malondialdehyde, it was attributed to oxidative stress.

کلیدواژه‌ها [English]

Oxidative stress
Angiogenesis
Mid-term swimming
Long-term swimming

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