نوع مقاله : علمی - پژوهشی

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، واحد یادگار امام خمینی (ره) شهر ری، دانشگاه آزاد اسلامی تهران، ایران

2 دانشکده تربیت بدنی، واحد علی آباد کتول، دانشگاه آزاد اسلامی، علی آباد کتول، ایران

چکیده

هدف: میتوفاژی می­تواند به کنترل کیفیت میتوکندری کمک کند. هدف از مطالعه حاضر، بررسی تأثیر یک دوره تمرین بر عوامل میتوفاژی میتوکندری در موش های صحرایی نر چاق بود.
روش‌ها: در این مطالعه تجربی، 40 سر موش صحرایی نر (میانگین وزن20±120 گرم) پس از القاء چاقی با رژیم غذایی پرچرب (به مدت 10 هفته)، 8 سر موش صحرایی از گروه رژیم غذایی پرچرب (O) و 8 سر موش صحرایی گروه رژیم غذایی استاندارد (C) برای بررسی القای چاقی تشریح شدند و سایر موش‌های صحرایی چاق به‌طور تصادفی به 3 گروه کنترل چاق (OC)، تمرین تداومی با شدت متوسط (MICT) و تمرین تناوبی با شدت بالا (HIIT) تقسیم شدند. HIIT شامل 10 مرتبه‌ فعالیت 4 دقیقه‌ای با شدت 90-85 درصد VO2max و دفعه های استراحت فعال 2 دقیقه‌ای و برنامه MICT نیز 5 جلسه در هر هفته با شدت 70-65 درصد VO2max به مدت 12 هفته اجرا شد. سطوح bcl2 و پارکین با استفاده از روش الکتروفورز ژل و وسترن بلات اندازه­گیری شد. داده­ها با استفاده از آزمون‌های تحلیل واریانس یک‌طرفه و آزمون تعقیبی توکی در سطح معنا‌داری 05/0P≤  تجزیه و تحلیل شد.
نتایج: نتایج نشان داد هر دو مداخله تمرین HIIT و MICT به افزایش معنا دار bcl2 و پارکین عضله نعلی نسبت به گروه کنترل منجر شدند (05/0P<). تفاوت معنا‌داری بین دو گروه HIIT و MICT در سطوح  bcl2و پارکین رت‌های چاق وجود نداشت (05/0<P).
نتیجه‌گیری: با توجه به نتایج، به نظر می‌رسد که HIIT و MICT می­توانند به کاهش تخریب و اختلال میتوکندری در عضله اسکلتی طی چاقی کمک کنند.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of training on mitochondrial mitophagy factors in obese male rats

نویسندگان [English]

  • Mozhgan Ahmadi 1
  • Neda Aghaei Bahman Begloo 2

1 Department of Physical Education and Sport Sciences Yadegar-e- Imam Khomeini (RAH) Shahr-e Rey Branch, Islamic Azad University, Tehran, Iran

2 Faculty of Physical Education, Aliabad Katoul Branch, Islamic Azad University, Aliabad Katoul Branch, Iran

چکیده [English]

purpose: Mitophagy can assist in mitochondrial quality control. The aim of this study was to investigate the effect of training on mitochondrial mitophagy factors in obese male rats.
Methods: In this experimental study, 40 male rats (weight 120 ± 20 g) after inducing obesity with high fat diet (for 10 weeks), eight rats from the high-fat diet group (O) and eight rats of the standard dietary group (C) to investigate the induction of obesity were described and other obese rats were randomly divided into three groups: obesity control (OC), moderate intensity continuous training (MICT) and high intensity interval training (HIIT). The HIIT protocol includes 10 bouts of 4-minute activity with intensity of 85-90% vo2max and 2-minute active rest periods and MICT protocols performed five sessions per week, with intensity of 65-70% VO2max for 12 weeks. Bcl2 and parkin levels were measured by gel electrophoresis and western blotting. Data were analyzed by One-way ANOVA and post hoc Tukey at P ≤ 0.05.
Results: The results showed that both HIIT and MICT training significantly increased bcl2 and PARKIN of Soleus muscle in comparison with the control group (P < 0.05). However, there was no significant difference between the two groups of HIIT and MICT in bcl2 and PARKIN levels of Soleus muscle in obese male rats (P > 0.05).
Conclusion: According to the results, it seems that HIIT and MICT can help reduce mitochondrial degradation and impairment in skeletal muscle during obesity.

کلیدواژه‌ها [English]

  • Interval training
  • continuous Training
  • bcl2
  • PARKIN
  • Obesity
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