نوع مقاله : علمی - پژوهشی

نویسندگان

دانشکده علوم ورزشی، دانشگاه مازندران، بابلسر، ایران

چکیده

هدف: اثرات سازگاری با ورزش بر بهبود چرخه پورین نوکلئوتیدها اجتناب ناپذیر است و فعالیت بدنی موجب بهبود مسیر بازیافت پورین ­ها (salvage) می­شود. تأثیر شیوه­ های مختلف تمرینی به ­ویژه نوع تناوبی خیلی شدید در فعالیت­های بسیار کوتاه مدت و دوره تمرینی کوتاه مدت به خوبی روشن نیست. پژوهش حاضر به منظور بررسی سازگاری احتمالی مسیر بازیافت پورین­ها از جمله آنزیم هیپوگزانتین –گوانین فسفوریبوزیل ترنسفراز (HGPRT)، هیپوگزانتین، گزانتین و همچنین اسید اوریک در یک دوره تمرینات تناوبی با شدت بالا طی دو هفته انجام شد.
روش­ها: در این پژوهش نیمه تجربی، هجده مرد سالم تمرین نکرده (میانگین و انحراف استاندارد سن34/2 ±95/21 سال و شاخص توده بدنی84/2 ±95/22 کیلوگرم بر متر مربع) برای این پژوهش داوطلب شدند و به ­صورت تصادفی در دو گروه کنترل و تمرین قرار گرفتند. تمرین شامل فعالیت­های 15 ثانیه­ای رکاب زدن با نهایت شدت بر روی چرخ ثابت و با استراحت 4 دقیقه­ای بین هر فعالیت بود که به مدت 2 هفته انجام شد. نمونه­ های خونی برای اندازه­ گیری متغیرهای ذکر شده، قبل از دوره و 48 ساعت پس از آخرین جلسه تمرین جمع آوری شد و داده­ها با استفاده روش آماری تحلیل کوواریانس تحلیل و آلفا در سطح 05/0 پذیرفته شد.
نتایج: افزایش معنا­داری در سطوح هیپو گزانتین (001/0P=)، گزانتین (001/0P=) و کاهش معنا­دار اسید اوریک (02/0P=) در گروه تمرین مشاهده شد و آنزیم HGPRT  بین دو گروه تفاوت معنا داری نداشت (386/0p=).
نتیجه­ گیری: نتایج نشان دهنده ی این بود که تمرینات تناوبی شدید موجب دفع بیشتر پایه­­های پورینی همانند هیپوگزانتین و گزانتین می­شود و کاهش اسید اوریک استراحتی احتمالاً به ­علت افزایش ظرفیت ضداکسایشی آن­ها می­باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Two Weeks of High-intensity Interval Training on Salvage nucleotide pathway

نویسندگان [English]

  • Abbas Ghanbari Niaki
  • Rouhollah Haghshenas Gatabi

Faculty of Sports Sciences, University of Mazandaran, Babolsar, Iran

چکیده [English]

Purpose: The effects of exercise adaptation on improving the purine nucleotides components are inevitable, and physical activity improves the purine nucleotide salvage pathway. The impact of different training methods, especially the very high intensity exercise and very short bouts and short training periods is not well understood. Therefore, the present study aims to investigate the possible compatibility of high intensity interval training on hypoxanthine, xanthine, hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and serum uric acid in a short-term.
Methods: In this semi-experimental study, eighteen healthy, untrained, male with mean and standard deviation were age 21.95 ± 2.34 years old and BMI 22.95 ± 2.84 kg/m2 eligible volunteers were randomly divided into control and training groups. The training group cycled the bicycle ergometer with maximum intensity for two weeks (three sessions per week) with 15-second repetitions and a 4-minute rest between the sets. Blood samples were collected for measuring HGPRT, hypoxanthine, xanthine and uric acid before and 48 hours after the last training session, and data were analyzed using analysis of covariance and Alpha level of 0.05.
Results: A significant increase was found in the levels of hypoxanthine (P = 0.001), xanthine (P = 0.001) and statistically significant reduction was found in uric acid (P = 0.02), and HGPRT enzyme was not significantly different the two groups.
Conclusion: The results of this study indicated that high intensity interval training causes the excretion of more purine bases such as hypoxanthine and xanthine, and reduce uric acid at rest is possibly increase antioxidant capacity.

کلیدواژه‌ها [English]

  • High-intensity interval training
  • HGPRT
  • Hypoxanthine
  • xanthine
  • uric acid
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