اثر دو ماه تمرین ترکیبی و محدودیت کالری بر مقدار P65 سلول‌های تک‌هسته‌ای خون محیطی مردان غیرفعال میانسال

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشکده علوم ورزشی و تندرستی، دانشگاه شهید بهشتی، تهران، ایران

2 دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه تبریز، تبریز، ایران

3 دانشگاه علوم پزشکی تبریز، تبریز، ایران

4 دانشگاه پیام نور، تهران، ایران

چکیده

هدف: شناسایی سازوکارهای درگیر در اختلالات التهابی طی فرایند افزایش سن و ارائۀ راهکارهای پیشگیرانه یا تعدیل‌کنندۀ مناسب برای بهبود کیفیت زندگی همواره از چالش‌های مهم در حوزۀ علوم زیستی است. بر این اساس، تحقیق حاضر با هدف تعیین اثر دو ماه تمرین ترکیبی (RT+HIIT) و محدودیت کالری بر شاخص‌های التهابی CRP سرمی و مقدار P65 در سلول‌های تک‌هسته‌ای خون محیطی مردان غیرفعال میانسال انجام گرفت
روش‌ها: 24 مرد میانسال غیرفعال و سالم (سن 3±38 سال) و دارای اضافه وزن (کیلوگرم بر متر مربع 1±6/28=BMI) در سه گروه همگن هشت‌نفری محدودیت کالری (CR)، تمرین ترکیبی (T) و تمرین ترکیبی با محدودیت کالری (CRT) شرکت کردند. محدودیت کالری و هزینۀ انجام تمرینات طی 5 روز در هفته حدود (شش کیلوکالری/کیلوگرم/روز) بود. تمرین ترکیبی شامل 5 روز در هفته (2 جلسه تمرین مقاومتی 75 تا 80% 1RM و 3 جلسه تمرین تناوبی با شدت بالا80 تا 85% ضربان قلب ذخیره) بود. مقدار p65 کل (total p65) و فسفریله (p-p65) در PBMCs مردان غیرفعال به روش الیزا پیش و پس از دورۀ پژوهش، اندازه‌گیری شد. غلظت CRP سرمی نیز به شیوۀ ایمونوتوربیدیمتریک اندازه‌گیری شد.
نتایج: کاهش وزن بدن گروه CRT به‌طور معناداری (05/0>P) بیشتر از دو گروه دیگر بود. دامنۀ تغییرات درصد چربی، اکسیژن مصرفی بیشینه و قدرت یک تکرار بیشینۀ گروه‌های T و CRT به‌طور معنادار (05/0>P) بیشتر از گروه CR بود. تغییرات کاهندۀ P65 فسفریله و نسبت p-p65/total p65 در PBMCهای گروه‌های T و CRT به‌طور معناداری (05/0>P) بیشتر از گروه CR بود.
نتیجه‌گیری: براساس نتایج تحقیق حاضر دو ماه تمرین ترکیبی و محدودیت کالری می‌تواند سبب کاهش برخی شاخص‌های التهابی در مردان غیرفعال شود. به‌علاوه، اثر تمرین ترکیبی با و بدون محدودیت کالری بر عوامل آغازگر فرایندهای التهابی بیشتر از مداخلۀ محدودیت کالری به‌تنهایی است. بنابراین، برای پیشگیری و مقابله با پدیدۀ چاقی و پیامدهای التهابی آن، به‌ویژه در دوران میانسالی، انجام تمرینات ترکیبی و محدودیت کالری توصیه می‌شود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of two-month concurrent training and caloric restriction on P65 amount in Peripheral blood mononuclear cells of inactive middle-aged men

نویسندگان [English]

  • Afshar Jafari 1
  • Farid Etemadian 2
  • Behzad Baradaran 3
  • Ali Akbar Malekirad 4
1 Faculty of Sports and Health Sciences, Shahid Beheshti University, Tehran, Iran
2 Faculty of Physical Education and Sports Sciences, University of Tabriz, Tabriz, Iran
3 Tabriz University of Medical Sciences, Tabriz, Iran
4 Payame Noor University, Tehran, Iran
چکیده [English]

 Purpose: Recognition of mechanisms involved in inflammatory disorders through the aging process and providing appropriate preventive or modulator strategies to improve quality of life are always important challenges in the field of life sciences. Therefore, the present study was conducted to determine the effect of two-month concurrent training (RT+HIIT) and caloric restriction on serum CRP and P65 amount in Peripheral blood mononuclear cells of inactive middle-aged men.
Methods: Twenty-four inactive, healthy men participated in three homogeneous groups: Caloric restriction (CR), Concurrent training (T) and Concurrent training with Caloric restriction (CRT) groups. Calorie restriction and energy cost in five days a week was about six kcal·kg-1·day-1. The concurrent training was included five days per week (two sessions of resistance training with 75 to 80% 1RM and three sessions of high intensity interval training with 80-85% heart rate reserve). Baseline and post-intervention p65 in the total and phosphorylated (p-p65) levels in PBMCs were assessed by ELISA. Serum CRP concentration was also measured using immunoturbidimetric.
Results: Weight loss in CRT group was significantly higher than the other two groups (P < 0.05). However, the changes range in fat percentage, maximal oxygen consumption and one-repetition maximum (1RM) of T and CRT groups were significantly higher than CR group (P < 0.05). Changes in phosphorylated P65 and p-p65 / total p65 ratio in PBMCs of T and CRT groups were significantlyhigher than CR group (P < 0.05).
Conclusion: Based on the present results, two months of concurrent training and caloric restriction can reduce some of inflammatory markers in inactive men. In addition, the effect of concurrent training with and without calorie restriction on the initiators of inflammatory factors is greater than the calorie restriction intervention alone. Therefore, concurrent training and caloric restriction are recommended for prevention and confronting obesity and its inflammatory consequences, especially in middle age.

کلیدواژه‌ها [English]

  • Concurrent training
  • Caloric restriction
  • P65
  • CRP

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سابقه مقاله

  • تاریخ دریافت: 19 مهر 1398
  • تاریخ بازنگری: 22 خرداد 1399
  • تاریخ پذیرش: 02 تیر 1399
  • تاریخ اولین انتشار: 01 مهر 1400
  • تاریخ انتشار: 01 مهر 1400

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