نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشکدة تربیت بدنی و علوم ورزشی، پردیس دانشگاه تهران، کیش، ایران

2 دانشکدة تربیت بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

چکیده

زمینه و هدف: شناسایی و تجزیه‌وتحلیل اجزایی که در به تأخیر انداختن پیری و افزایش طول عمر نقش دارند، بسیار ضروری است. سیرتوئین‌ها از تنظیم‌کننده‌های اصلی بقای سلولی و طول عمر هستند. هدف پژوهش حاضر بررسی تأثیر شش هفته فعالیت ورزشی HIIT شنا و مکمل رزوراترول بر مقدار SIRT3 در بافت بطن چپ قلب موش‌های صحرایی پیر بود.
مواد و روش‌ها: در این پژوهش، 30 سر موش صحرایی 20 ماهه (پیر) نر نژاد ویستار تصادفی به پنج گروه (کنترل (C)، گروه تمرین (EX)، گروه تمرین+مکمل (EXR)، گروه مکمل (R) و گروه حلال (M)) تقسیم شدند. گروه EX، تمرین HIIT  شنا را به مدت شش هفته انجام دادند. گروه C، تمرین نمی‌کردند. گروه R، فقط مکمل رزوراترول دریافت کردند. گروه EXR، تمرین HIIT  شنا را همراه با دریافت رزوراترول انجام دادند. گروه M فقط حلال دریافت کردند. SIRT3 با استفاده از روش آزمایشگاهی وسترن بلات سنجیده شد. داده‌های حاصله توسط آزمون آماری تحلیل واریانس یکطرفه و آزمون تعقیبی توکی تجزیه‌وتحلیل شدند.
نتایج: پس از شش هفته، تفاوت معناداری بین میانگین میزان پروتئین SIRT3 در پنج گروه مشاهده شد. بنابراین، آزمون تعقیبی توکی نشان داد که مقدار SIRT3 در گروه EX و EXR در مقایسه با گروه C، R و M، به‌طور معناداری بیشتر بود (001/0P=)، اما این تفاوت بین دو گروه EX و EXR معنادار نبود (05/0P>). همچنین، مقدار SIRT3 در گروه R به‌طور معناداری در مقایسه با گروه C و M بیشتر بود.
نتیجه‌گیری: افزایش SIRT3، نشان‌دهندة دخیل بودن این پروتئین در مسیرهای سوخت‌وسازی، دفاع ضداکسایشی و بهبود وضعیت سلول است. همچنین می‌توان از تمرین تناوبی شدید همراه با مصرف مکمل رزوراترول برای تعدیل فشار اکسایشی و تأخیر روند پیری و سالمندی بهره برد.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of six weeks High Intensity Interval Swimming Training and Resveratrol supplementation on the level of SIRT3 in left ventricular heart of aged rats

نویسندگان [English]

  • Ali Reza Rezaei 1
  • Abbas Ali Gaeini 2
  • Sirous Choobineh 2
  • Reza Nuri 1

1 Faculty of Physical Education and Sport Sciences, University of Tehran Kish International Campus, Kish, Iran

2 Faculty of Physical Education and Sport Sciences, University of Tehran, Tehran, Iran

چکیده [English]

Background and Purpose: Identification and analysis of compounds that playing a role in delay aging and increase longevity is essential. Sirtuins are one of the main regulators of cell survival and longevity. The goal of this research was to investigate the effect of six weeks HIIT swimming exercise and Resveratrol supplementation on the level of SIRT3 in left ventricular heart of aged rats.
Materials and Methods: In this research, 30 wistar rats were randomly divided into five groups (control (C), solvent (M), supplement (R), HIIT exercise (EX), HIIT exercise and supplement (EXR)). The EX group performed HIIT swimming for six weeks. C group did not practice. R group received only resveratrol supplementation. EXR group performed HIIT swimming exercises with resveratrol and M group received only solvent. The SIRT3 protein was obtained by Western blotting. Data were analyzed using One-way ANOVA analysis of variance and Tukey's post hoc test.
Results: After six weeks, there was a significant difference between the mean SIRT3 protein levels in the five groups. Therefore, Tukey post hoc test showed that SIRT3 levels were significantly increased in EX and EXR groups compared to C, R and M groups (P = 0/001). However, this difference between EX and EXR groups was not significant (P > 0.05). Moreover, the amount of SIRT3 in group R was significantly higher compared to groups C and M.
Conclusion: Increasing the amount of SIRT3 indicates the involvement of this protein in metabolic pathways, antioxidant defense and improves cell condition. High intensity interval training with resveratrol supplement consumption can also be used to moderate oxidative stress and delay the aging process.

کلیدواژه‌ها [English]

  • Life span
  • Exercise training
  • Antioxidant Supplements
  1. Cui, Hang, Yahui Kong, and Hong Zhang. Oxidative stress, mitochondrial dysfunction, and aging. Journal of signal transduction.2012;(2012)1-13.
  2. Shin, Jung-Won, et al. Molecular mechanisms of dermal aging and antiaging approaches. International journal of molecular sciences. 2019;20(9).21-26.
  3. Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR. Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice. J Proteome Res. 2012;11(3):1633-1643.
  4. Guarente L, Picard F. Calorie restriction the SIR2 connection. Cell. 2005;120(4):473-82.
  5. Vaziri H, Dessain SK, Ng Eaton E, Imai SI, Frye RA, Pandita TK, Guarente L, Weinberg RA. hSIR2 (SIRT1) functions as an NAD-dependent p53 deacetylase. 2001;107(2):149-59.
  6. Corbi, G, Conti, V, Scapagnini, G, Filippelli, A, Ferrara N. Role of sirtuins, calorie restriction and physical activity in aging. Front Biosci (Elite Ed).2012;768-778.
  7. McDonnell E, Peterson BS, Bomze HM, Hirschey MD. SIRT3 regulates progression and development of diseases of aging. Trends Endocrinol Metab. 2015;26(9):486-492.
  8. Winnik S, Auwerx J, Sinclair DA, Matter CM. Protective effects of sirtuins in cardiovascular diseases: from bench to bedside. European heart journal. 2015;36(48):3404-12.
  9. Palacios OM, Carmona JJ, Michan S, Chen KY, Manabe Y, Ward III JL, Goodyear LJ, Tong Q. Diet and exercise signals regulate SIRT3 and activate AMPK and PGC-1α in skeletal muscle. Aging (Albany NY). 2009;1(9):771.
  10. Singh SP, Schragenheim J, Cao J, Falck JR, Abraham NG, Bellner L. PGC-1 alpha regulates HO-1 expression, mitochondrial dynamics and biogenesis: Role of epoxyeicosatrienoic acid. Prostaglandins Other Lipid Mediat. 2016;125:8-18
  11. Cheng A, Yang Y, Zhou Y, Maharana C, Lu D, Peng W, Liu Y, Wan R, Marosi K, Misiak M, Bohr VA, Mattson MP. Mitochondrial SIRT3 Mediates Adaptive Responses of Neurons to Exercise and Metabolic and Excitatory Challenges. Cell Metab. 2016;23(1):128-42.
  12. Green MF, Hirschey MD. SIRT3 weighs heavily in the metabolic balance: a new role for SIRT3 in metabolic syndrome. J Gerontol A Biol Sci Med Sci. 2013;68(2):105-7.
  13. Knutson MD, Leeuwenburgh C. Resveratrol and novel potent activators of SIRT1: effects on aging and age-related diseases. Nutrition reviews. 2008;66(10):591-6.
  14. Li H, Xia N, Forstermann U. Cardiovascular effects and molecular targets of resveratrol. Nitric Oxide. 2012;26(2):102-10.
  15. Li H, Xia N, Hasselwander S, Daiber A. Resveratrol and Vascular Function. Int J Mol Sci. 2019;20(9):2155.
  16. Chen T, Li J, Liu J, Li N, Wang S, Liu H, Zeng M, Zhang Y, Bu P. Activation of SIRT3 by resveratrol ameliorates cardiac fibrosis and improves cardiac function via the TGF-β/Smad3 pathway. American Journal of Physiology-Heart and Circulatory Physiology. 2015;308(5):424-34.
  17. Tyagi S, Sharma A., Aggarwal G. Clinical and medicinal application of resveraterol: a reviwe. International Journal of Pharmaceutical Sciences Review and Research 2010;3(1): 49-52.
  18. Gurd BJ, Perry CG, Heigenhauser GJ, Spriet LL, Bonen A. High-intensity interval training increases SIRT1 activity in human skeletal muscle. Appl Physiol Nutr Metab. 2010;35(3):350-7.
  19. Hokari, F., Kawasaki, E., Sakai, A., Koshinaka, K., Sakuma, K. and Kawanaka, K. Muscle contractile activity regulates Sirt3 protein expression in rat skeletal muscles. Journal of applied physiology.2010;109(2):332-340.
  20. Huang CC, Wang T, Tung YT, Lin WT. Effect of Exercise Training on Skeletal Muscle SIRT1 and PGC-1α Expression Levels in Rats of Different Age. Int J Med Sci. 2016;13(4):260-70.
  21. Dutt Way W, Soga T, Parhar IS. Aging and chronic administration of serotonin-selective reuptake inhibitor citalopram upregulate Sirt4 gene expression in the preoptic area of male mice. Frontiers in genetics. 2015;6:281.
  22. Walsh RN, Cummins RA. The open-field test: a critical review. Psychological bulletin. 1976;83(3):482.
  23. Ramos-Filho D, Chicaybam G, de-Souza-Ferreira E, Guerra Martinez C, Kurtenbach E, Casimiro-Lopes G, Galina A. High intensity interval training (HIIT) induces specific changes in respiration and electron leakage in the mitochondria of different rat skeletal muscles. PloS one. 2015;29;10(6):e0131766.
  24. Shafiee A, Gaeini A, Soleimani M, Nekouei A, Hadidi V. The effect of eight week of high intensity interval training on expression of mir-210 and ephrinA3 mRNA in soleus muscle healthy male rats. Journal of Arak University of Medical Sciences. 2014;17(3):26-34. )In Persian(.
  25. Fathi I, Noorshahi M, Haghparast A, Fallah Hoseini H. Effect of eight-week aerobic continuous and high intensity interval training on levels of SIRT3 in skeletal muscle tissue of Wistar rats. Journal of Sport and Exercise Physiology. 2015;8(2): 1277–1289.(In Persian).
  26. Mehrabi A, Gaeini AA, Nuri R, Daryanoosh F. The Effect of Six-Week HIIT Swimming Exercise and Resveratrol Supplementation on the Level of SIRT3 in Frontal Lobe of aged rats.the neuroscience journal of shefaye khatam.2021,9(2).48-59.
  27. Lanza IR, Short DK, Short KR, Raghavakaimal S, Basu R, Joyner MJ, McConnell JP, Nair KS. Endurance exercise as a countermeasure for aging. Diabetes. 2008;57(11):2933-42.
  28. M Xiao, X Xu, J Lu. Effect of High-intensity Interval Training and Accumulated Exercise on PGC-1α and SIRT3 Levels in the Skeletal Muscle of Rats and Bioinformatics Analysis, International Conference on Information Technology and Contemporary Sports (TCS), 2021.215-220.
  29. Chen D, Bruno J, Easlon E, Lin S-J, Cheng H-L, Alt FW, et al. Tissue-specific regulation of SIRT1 by calorie restriction. Genes & development.2008;22(13): 1753-7.
  30. Ahn BH, Kim HS, Song S, Lee IH, Liu J, Vassilopoulos A, Deng CX, Finkel T. A role for the mitochondrial deacetylase Sirt3 in regulating energy homeostasis. Proceedings of the National Academy of Sciences. 2008;105(38):14447-52.
  31. Little JP, Safdar A, Wilkin GP, Tarnopolsky MA, Gibala MJ. A practical model of low‐volume high‐intensity interval training induces mitochondrial biogenesis in human skeletal muscle: potential mechanisms. The Journal of physiology. 2010;588(6):1011-22.
  32. Hodge T, Starnes J, Feger B, Hixson L, Harris MB. Effects of exercise and body temperature on eNOS, SIRT1, SIRT3 and Hsp70 expression in rat plantaris muscles. The FASEB Journal. 2014;28:1164-6.
  33. Afzalpour MS, Sarir H, Zanjirian Z, Mohammadnia Ahmadi M, Ghasemi E. The effect of vigorous continuous and interval exercise training along with resveratrol on SIRT3 and OGG1 proteins in the liver tissue of male Wistar rats. Journal of Sport and Exercise Physiology; 2020:13(1):111-127.(In Persian).
  34. Desquiret-Dumas V, Gueguen N, Leman G, Baron S, Nivet-Antoine V, Chupin S, Chevrollier A, Vessières E, Ayer A, Ferré M, Bonneau D. Resveratrol induces a mitochondrial complex I-dependent increase in NADH oxidation responsible for sirtuin activation in liver cells. Journal of Biological Chemistry. 2013;288(51):36662-75.
  35. Singh B, Shoulson R, Chatterjee A, Ronghe A, Bhat NK, Dim DC, Bhat HK. Resveratrol inhibits estrogen-induced breast carcinogenesis through induction of NRF2-mediated protective pathways. Carcinogenesis. 2014;35(8):1872-80.
  36. Gertz M, Nguyen GT, Fischer F, Suenkel B, Schlicker C, Fränzel B, Tomaschewski J, Aladini F, Becker C, Wolters D, Steegborn C. A molecular mechanism for direct sirtuin activation by resveratrol. PloS one. 2012;7(11):e49761.