اثر هشت هفته تمرین تناوبی شدید همراه با محدودیت کالریک و مکمل اسپرولینا بر عوامل التهابی در بافت قلب موش‌های صحرایی تحت رژیم غذای پرچرب

نوع مقاله : علمی - پژوهشی

نویسندگان

1 گروه تربیت‌بدنی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج، ایران

2 گروه تربیت بدنی، واحد سنندج ، دانشگاه آزاد اسلامی ، سنندج، ایران

3 3. گروه تربیت بدنی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج

4 گروه تربیت بدنی، واحد سنندج، دانشگاه آزاد اسلامی، سنندج

چکیده

زمینه و هدف: افزایش عوامل التهابی متعاقب چاقی از دلایل اصلی مرگ‌ومیر است. اگرچه نقش تمرینات ورزشی، محدودیت کالریک و مکمل‌های ضداکسایشی در این زمینه گزارش شده است، اثر همزمان هر سه مداخله مورد توجه پژوهشگران زیادی است. ازاین‌رو هدف پژوهش حاضر بررسی اثر هشت هفته تمرین تناوبی شدید، محدودیت کالری و مکمل اسپرولینا بر عوامل التهابی در بافت قلب موش‌های صحرایی تحت رژیم غذای پرچرب بود.
مواد و روش‌ها: 45 سر موش صحرایی نر چاق‌شده با رژیم غذایی (60 درصد چربی، 20 درصد پروتئین و 20 درصد کربوهیدرات) به‌طور تصادفی در گروه‌های 1. رژیم غذایی با کالری بالا (HFD)، 2. مکمل اسپرولینا (Sp)، 3. محدودیت کالریک (CR)، 4. تمرینات تناوبی شدید (HIIT)، 5. مکمل اسپرولینا - تمرینات تناوبی شدید (HIIT+Sp)، 6. تمرینات تناوبی شدید - محدودیت کالریک (HIIT+CR)، 7. مکمل اسپرولینا - محدودیت کالریک (CR+Sp)، 8. تمرینات تناوبی شدید - محدودیت کالریک - مکمل اسپرولینا (HIIT+CR+Sp)، قرار داده شدند. همچنین پنج سر موش صحرایی نر سالم برای بررسی آثار رژیم غذایی با کالری بالا بر متغیرهای پژوهش در گروه کنترل سالم قرار گرفتند. تمرینات تناوبی شدید به مدت هشت هفته، سه جلسه در هفته با شدت 80-110درصد بیشینه سرعت دویدن و با 2 تا 8 تناوب با شدت بالا انجام گرفت، مصرف مکمل اسپرولینا به مدت هشت هفته mg/kg/day 100 به‌صورت خوراکی تجویز شد و گروه‌های محدودیت کالریک تنها 60 درصد از غذایی مصرفی در روز قبل را استفاده کردند. به‌منظور تجزیه‌وتحلیل داده‌ها از آزمون تحلیل واریانس یکراهه همراه با آزمون تعقیبی توکی استفاده شد (001/0≥P).
نتایج: مقادیر P38MAPK، NF-kB و TNF-α در گروه HIIT بیشتر و در گروه CR به‌طور معناداری کمتر از گروه HFD بود (001/0≥P). مقادیر TNF-α در گروه‌های Sp، HIIT+Sp و HIIT+CR به‌طور معناداری کمتر از گروه HFD بود (001/0≥P )؛ مقادیر NF-kB در گروه HIIT+CR به‌طور معناداری کمتر از HFD بود (001/0≥P ). همچنین در گروه HIIT+CR+Sp مقادیر NF-kB، TNF-α به‌طور معناداری کمتر و P38MAPK به‌طور معناداری بیشتر از گروه HFD بود (001/0≥P ).
نتیجه‌گیری: به‌نظر می‌رسد محدودیت کالریک و مکمل اسپرولینا به‌تنهایی تأثیر ضدالتهابی دارند، هرچند تأثیر ضدالتهابی محدودیت کالریک تا حدی بیشتر از اسپرولینا و HIIT بود. بنابراین استفاده از این دو مداخله در کنار تمرینات تناوبی شدید بر کاهش عوامل التهابی متعاقب چاقی و اضافه وزن در نمونه‌های حیوانی مطلوب است. 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of eight weeks of high-intensity interval training with caloric restriction and spirulina supplementation on inflammatory factors in heart tissue of rats under high-fat diet

نویسندگان [English]

  • Jamshid Khirabadi 1
  • Zaher Etemad 2
  • Kamal Azizbeigi 3
  • khalid mohamadzadeh salamat 4
1 Department of exercise Physiology Islamic Azad University Sanandaj Sanandaj Iran
2 Assistant Professor, Department of Exercise Physiology, Islamic Azad University, Sanandaj Branch,, Sanandaj, Iran.
3 Associate Professor Department of ٍExercise Physiology Islamic Azad University, Sanandaj Sanandaj Branch, Sanandaj Iran
4 Assistant Professor, Department of Physical Education, Sanandaj Branch, Islamic Azad University, Sanandaj, Ira
چکیده [English]

Background and Purpose: The increase of inflammatory factors following obesity is known as one of the main causes of mortality. Although the role of exercise training, caloric restriction and antioxidant supplements have been reported in this field; But their simultaneous effect is of interest to many researchers. Therefore, the aim of the present study was to investigate the effect of eight weeks of HIIT (High-intensity interval training) , calorie restriction and spirulina supplementation on inflammatory factors in the heart tissue of rats fed a high-fat diet.
Materials and Methods: 45 obese male rats with a diet (60% fat, 20% protein, and 20% carbohydrate) were randomly divided into eight groups (1) high-calorie diet (HFD) (2) spirulina supplement (Sp), (3) caloric restriction (CR), (4) intense interval training (HIIT), (5) spirulina supplement - intense interval training (HIIT+Sp), (6) intense interval training - caloric restriction (CR+HIIT), (7) spirulina supplement-caloric restriction (CR+Sp), (8) intense interval training-caloric restriction-spirulina supplement (CR+HIIT+Sp). Also, five healthy rats were included in the healthy control group to investigate the effects of a high-calorie diet on the research variables. Intense interval training was performed for eight weeks, three sessions per week with an intensity of 80-110% of the maximum running speed and with 2-8 high intensity intervals, spirulina supplement 100 mg/kg/day was administered orally for eight weeks. Moreovwe, the caloric restriction groups used only 60% of the food consumed the previous day. To analyze the data, one-way analysis of variance was used along with Tukey's post hoc test (P ≤ 0.001).
Results: The values of P38MAPK, NF-κB and TNF-α were higher in the HIIT group and significantly lower in the CR group than the HFD group (P ≤ 0.001). The levels of TNF-α in the spirulina supplement, spirulina supplement-intense central training and intense interval training-caloric restriction groups were significantly lower than the high-calorie diet group (P ≤ 0.001); The values ​​of NF-kB in the group of intense interval training-caloric restriction were significantly lower than those of the high-calorie diet (P ≤ 0.001). Also, in the severe interval marinade-caloric restriction-spirulina supplement group, NF-kB, TNF-α values ​​were significantly lower and P38MAPK was significantly higher than the high-calorie diet group (P≤0.001).
Conclusion: It seems that caloric restriction and spirulina supplement alone have anti-inflammatory effects, although the anti-inflammatory effect of caloric restriction was somewhat higher than that of spirulina and HIIT; Therefore, the use of these two interventions, along with HIIT, is desirable for reducing inflammatory factors following obesity and overweight in animal models.

کلیدواژه‌ها [English]

  • intense interval training
  • spirulina
  • caloric restriction
  • inflammation factor

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سابقه مقاله

  • تاریخ دریافت: 25 شهریور 1401
  • تاریخ بازنگری: 23 آبان 1401
  • تاریخ پذیرش: 28 آذر 1401
  • تاریخ اولین انتشار: 01 فروردین 1402
  • تاریخ انتشار: 01 فروردین 1402

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