اثر تمرین مقاومتی بر بازیافت سطح تستوسترون سرمی و برخی از شاخص‌های تولید مثلی پس از مصرف آسپرین در موش‌های صحرایی نر بالغ

نوع مقاله : علمی - پژوهشی

نویسندگان

گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه شاهد. تهران، ایران

چکیده

زمینه و هدف: بررسی دلایل ایجادکنندة ناباروری در مردان و چگونگی جلوگیری از ایجاد ناباروری در جامعه، کمک ارزنده به بیمارانی است که از ناباروری رنج می‌برند. ناباروری مردان به علل گوناگونی از جمله سن، داروها، قرار گرفتن در معرض سموم محیطی، مشکلات ژنتیکی و بیماری‌های عمومی رخ می‌دهد. آسپرین (استیل سالیسیلک اسید: ASA) یکی از داروهای ضدالتهابی غیراستروئیدی (NASID) و جزء پرمصرف‌ترین داروهای جهان است که سبب کاهش توان باروری مردان می‌شود. علاوه‌بر این فعالیت بدنی می‌تواند بر ناباروری جنس نر تأثیر بگذارد. هدف از پژوهش حاضر بررسی اثر تمرین مقاومتی بر سطح تستوسترون سرمی و برخی از مهم‌ترین شاخص‌های تولیدمثلی در موش‌های صحرایی بالغ نر ویستار مصرف‌کنندة آسپرین بود.
مواد و روش‌ها: روی‌هم‌رفته 25 سر موش صحرایی بالغ نر ویستار به‌طور تصادفی به دو گروه کنترل (10=n،C) و گروه مصرف دارو (15=n،AS) تقسیم و جایگزین شدند. پس از پنج هفته، پنج سر موش صحرایی از هر گروه قربانی شده و اثر مصرف آسپرین بر سطح تستوسترون سرمی و شاخص‌های تولیدمثلی نر بررسی شد. پس از گذشت دورة مصرف دارو و تأیید اثر داروی آسپرین، مصرف آن قطع و موش‌های صحرایی باقی‌مانده وارد دورة تمرین شدند. در دورة تمرین، 10 سر موش صحرایی باقیمانده، به‌صورت تصادفی از گروه مصرف دارو به گروه بدون مداخله (5=n، NI) و گروه تمرین مقاومتی (5=n،RT) جایگزین شدند. تمرین مقاومتی سه جلسه در هفته و به مدت چهار هفته انجام گرفت. 24 ساعت پس از آخرین جلسة تمرین، موش‌های صحرایی قربانی و نمونه‌های سرم و بافت برداشته شد. برای تجزیه‌وتحلیل تفاوت بین‌گروهی در دورة مصرف دارو از آزمون t-test استفاده شد. در دورة تمرین داده‌هــا بــا اســتفاده از آزمــون تحلیــل واریانــس یکطرفه و آزمــون تعقیبــی توکــی در سـطح معنـاداری کوچک‌تر از 05/0 در نرم‌افزار SPSS24 بررسـی شـد.
نتایج: یافته‌ها نشان داد که پنج هفته مصرف آسپرین به کاهش معنادار در سطح تستوسترون سرم و تعداد سلول‌های لیدیگ منجر شده است (05/0˂P). افزون‌بر این تعداد، تحرک، زنده ماندن اسپرم، شاخص تمایز لوله‌ای (Tubular DifferentiationIndex TDI:)، شاخص اسپرمیوژنز (Spermiogenesis Index (SI:، ضریب تجمعی (RI: Repopulation Index) و افزایش معناداری در هستة نابالغ و تکه‌تکه شدن DNA اسپرم شده بود (001/0 ˂P). همچنین چهار هفته تمرین مقاومتی در موش‌های صحرایی گروه RT تغییرات منفی مصرف داروی آسپرین را به‌طور معناداری نسبت به گروه NI بهبود بخشید و بازیافت این تغییرات در حد گروه CI را تسریع کرد (05/0 ˂P). روی‌هم‌رفته تغییرات معناداری در تحرک اسپرم بین گروه‌های پژوهش دورة تمرین مشاهده نشد (05/0<P).
نتیجه‌گیری: نتایج پژوهش حاضر تغییرات مضر داروی آسپرین بر هورمون تستوسترون و شاخص‌های تولیدمثلی جنس نر را تأیید می‌کند. افزون‌بر این یافته‌ها نشان داد که ممکن است تمرین مقاومتی سبب بهبود و بازیافت اثر مضر آسپرین بر شاخص‌های مرتبط با دستگاه تولیدمثلی جنس نر شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of resistance training on the recovery of serum testosterone levels and some reproductive parameters after aspirin consumption in adult male rats

نویسندگان [English]

  • Milad Khosravi sadr
  • Esmail Nasiri
Department of Physical Education and Sports Sciences, Faculty of Humanities, Shahed University. Tehran Iran
چکیده [English]

Background and Purpose: Studying the causes of infertility and appropriate interventions to protect male fertility is a valuable help to patients who suffer from infertility. There are various causes that can lead to male fertility disorder, including age, drugs, exposure to environmental toxins, genetic problems, and systemic diseases. Aspirin (acetylsalicylic acid: ASA) is one of the most widely used nonsteroidal anti-inflammatory drugs (NASID) in the world, which reduces male fertility. In addition, physical activity can affect male infertility. The purpose of this study was to investigate the effect of resistance training on serum testosterone levels and some of the most important reproductive parameters in adult male Wistar rats consuming ASA.
Materials and Methods: A total of twenty-five adult male Wistar rats were randomly divided into control (n=10, C) and the drug administration groups (n=15, AS). After five weeks, five rats from each group were sacrificed and the effect of ASA consumption on serum testosterone level and male reproductive parameters was investigated. After the period of taking the drug and confirming the impact of the ASA drug, its use was stopped and the remaining rats entered the training period. During the training period, the remaining 10 rats were randomly divided from the AS group into the non-intervention group (n=5, NI) and the resistance training group (n=5, RT). Resistance training was performed three times a week for four weeks. Twenty-four hours after the last training session, rats were sacrificed and serum and tissue samples were collected. t-test was used to analyze the difference between groups in the period of drug use. In the training period, the data were analyzed using a one-way analysis of variance and Tukey's post hoc test at a significance level of less than 0.05 in SPSS 24 software.
Results: The findings showed that five weeks of ASA consumption led to a significant decrease in serum testosterone levels and cell counts. (P < 0.05) Also, the number, motility, viability of sperm, TDI, SI, RI, as well as a significant increase in the immature nucleus and fragmentation of sperm DNA were abiserved.(P < 0.001). In addition, it was observed that four weeks of resistance training in RT group rats significantly improved the negative changes of ASA drug use compared to the NI group and accelerated the recovery of these changes to the level of the CI group (P < 0/05). However, no significant changes in sperm motility were observed between the research groups during the training period (P > 0.05).
Conclusion: The results of the present study confirm the harmful changes of ASA drug on testosterone hormone and male reproductive parameters. In addition, the findings showed that resistance training may improve and recover the harmful effect of ASA on the parameters related to the male reproductive system.

کلیدواژه‌ها [English]

  • : Resistance training
  • Aspirin
  • Spermatogenesis
  • Sperm
  • Rat

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سابقه مقاله

  • تاریخ دریافت: 28 مهر 1401
  • تاریخ بازنگری: 14 دی 1401
  • تاریخ پذیرش: 03 بهمن 1401
  • تاریخ اولین انتشار: 01 فروردین 1402
  • تاریخ انتشار: 01 فروردین 1402

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