تأثیر مصرف عصارة عناب طی دو نوع تمرین تناوبی و استقامتی شدید بر فشار اکسایشی و شاخص‌های ضداکسایشی بافت کبد موش‌های صحرایی نر ویستار نابالغ

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، زنجان، ایران

2 کارشناسی ارشد فیزیولوژی ورزشی کاربردی، گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، زنجان، ایران

3 دانشیار گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه زنجان، زنجان، ایران

چکیده

زمینه و هدف: یکی از راهکارهای مناسب برای محافظت در برابر تأثیرات نامطلوب فشار اکسایشی ناشی از تمرینات ورزشی طولانی‌مدت و شدید، به‌کارگیری تدابیر تغذیه‌ای سالم و استفاده از مکمل‌های ضداکسایشی است. هدف این پژوهش بررسی اثر مصرف عصارة عناب طی دو نوع تمرین تناوبی و استقامتی شدید بر شاخص‌های ضداکسایشی و فشار اکسایشی بافت کبد موش‌های صحرایی نابالغ نر بود.
مواد و روش‌ها: 72 سر موش نابالغ صحرایی نژاد ویستار با سن 22 روز و میانگین وزنی 475/7 ± 64/60 گرم پس از وزن‌کشی به‌صورت تصادفی به نه گروه کنترل، تمرین تناوبی شدید (HIT)، تمرین استقامتی شدید (ICT)، عناب، ICT + عناب، ICT + دارونما، HIT + دارونما، HIT + عناب و دارونما تقسیم شدند. پس از یک هفته آشناسازی عمومی و اختصاصی، برنامة تمرین استقامتی شدید شامل دویدن روی نوار گردان به مدت چهار هفته (پنج جلسه در هفته) با مدت و شدت پیش‌رونده و با رعایت اصل اضافه‌بار تمرین اجرا شد. به‌طوری‌که سرعت در هفتة اول برابر 10 تا 18 متر در دقیقه و مدت آن 10-30 دقیقه بود و در هفتة آخر سرعت تمرین به 30 متر بر دقیقه و مدت آن 55-60 رسید. برنامة تمرینی  HITنیز در قالب تمرین با الگوی دوره‌های هفت‌روزه (شش روز تمرین و یک روز استراحت به‌منظور جلوگیری از بیش‌تمرینی) انجام گرفت. بر اساس اصل اضافه‌بار تمرین به‌گونه‌ای اعمال شد که سرعت تمرین در هفتة آشناسازی برابر با 10-16 متر در دقیقه و مدت آن یک دقیقه بود و در هفتة آخر سرعت تمرین 36-40 متر در دقیقه (معادل حدود 85% Vo2max) رسید. به گروه‌های دارونما، گاواژ آب مقطر و به گروه‌های دارای عناب، گاواژ عصارة عناب با دوز 600 میلی‌گرم به ازای هر کیلوگرم وزن بدن هر موش، روزانه پس از تمرین ارائه شد. اندازه‌گیری میزان فعالیت آنزیم‌های SOD ، GPX و CAT و سطوح MDA و TAC بافت کبد به روش اسپکتروفتومتری و کیت الایزا انجام گرفت. برای تجزیه‌وتحلیل داده‌ها از روش‌های آماری آنوا و آزمون تعقیبی توکی استفاده شد.
نتایج: نتایج نشان داد که فعالیت استقامتی شدید و HIT موجب کاهش معنادار میزان MDA شد (05/0P<). هرچند بر میزان فعالیت آنزیم‌های ضداکسایشی SOD، GPX و سطوح TAC تأثیر معناداری نداشت (05/0P>). از سویی، تمرین HIT موجب افزایش معنادار آنزیم CAT شد (05/0P<). در گروه‌های دارای گاواژ عناب، تأثیر معناداری بر میزان آنزیم‌های ضداکسایشی SOD، GPX، CAT، TAC و MDA نداشتند (05/0P>).
نتیجه‌گیری: به‌نظر می‌رسد که تمرینات استقامتی شدید و HIT موجب تعدیل شاخص‌های اکسایشی و همچنین تمرین HIT، سبب بهبود نشانگر ضداکسایشی CAT در موش‌های نابالغ می‌شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

The effect of jujube extract consumption during two types of high intensity interval training and intensive endurance training on oxidative stress and antioxidant indices of liver tissue in immature male Wistar rats

نویسندگان [English]

  • Leila Youzbashi 1
  • Parisa Abdollahi 2
  • Ahmad Rahmani 3
  • Akram Karimiasl 1
1 Assistant Professor, Department of Sport Siences, Faculty of Humanities, University of Zanjan, Zanjan, Iran
2 MA Applied Exercise Physiology, Department of Sport Siences, Faculty of Humanities, University of Zanjan, Zanjan, Iran
3 Associate Professor, Department of Sport Siences, Faculty of Humanities, University of Zanjan, Zanjan, Iran
چکیده [English]

Background and Purpose: One of the appropriate strategies for combating the adverse effects of oxidative stress caused by strenuous activities is consuming a well-balanced diet and taking antioxidant supplements. The purpose of this study was to investigate the effect of jujube extract consumption during two types of intermittent and intense endurance training on antioxidant indices and oxidative stress of the liver tissue of immature male rats.
Materials and Methods: 72 male Wistar rats (aged= 22 days, weight= 60.64 ± 7.475 g) were randomly divided into nine groups after being weighed: control, placebo, jujube, intense endurance training (ICT), ICT + placebo, ICT + jujube gavage, high-intensity interval training (HIT), HIT + placebo gavage, HIT + jujube gavage. After a week of general and specific familiarization, the ICT protocol including running on the treadmill for four weeks (five sessions per week) with progressive duration and intensity and following the principle of exercise overload was implemented on an animal treadmill. So that the speed in the first week was equal to 10-18 meters per minute and its duration was 10-30 minutes, and in the last week the training speed reached 30 meters per minute and its duration was 55-60 minutes. The HIT training protocol was also carried out in the form of training with the pattern of seven-day courses (six days of training and one day of rest to prevent overtraining). Based on the principle of overload, training was applied in such a way that the training speed in the familiarization week was equal to 10-16 meters per minute and its duration was one minute, and in the last week the training speed reached 36-40 meters per minute (equivalent to about 85% of Vo2max). Distilled water was given to the placebo groups, and 600 mg/kg of jujube extract was given daily to the jujube groups after training. The activity of GPX, SOD, CAT enzymes, TAC and MDA was assayed by spectrophotometric method using diagnostic ELISA kit. ANOVA and Tukey's post hoc test were used to analyze the data.
Results: The results showed that ICT and HIT significantly decreased MDA activity (P < 0.05). However, ICT and HIT did not affect the activity levels of antioxidant enzymes (SOD, GPX) and TAC (P > 0.05). On the other hand, HIT training significantly increased CAT enzyme (P < 0.05). The results also showed that groups with jujube gavage did not have a significant effect on the activity levels of antioxidant enzymes (SOD, GPX, CAT), TAC and MDA (P > 0.05).
Conclusion: it seems that ICT, HIT moderates oxidative indices, and HIT training improves the antioxidant index CAT in immature rats.

کلیدواژه‌ها [English]

  • Intensive Endurance Training
  • High Intensity interval training
  • Antioxidant
  • Malondialdehyde
  • Total antioxidant capacity
  • Jujube extract

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  • تاریخ دریافت: 25 مهر 1401
  • تاریخ بازنگری: 16 دی 1401
  • تاریخ پذیرش: 26 بهمن 1401
  • تاریخ اولین انتشار: 01 فروردین 1402
  • تاریخ انتشار: 01 فروردین 1402

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