نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه فیزیولوژی ورزشی ، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران
2 گروه علوم پایه ، دانشکده دامپزشکی ، دانشگاه شهید چمران اهواز، اهواز ، ایران
3 1. گروه فیزیولوژی ورزشی ، دانشکده علوم ورزشی، دانشگاه شهید چمران اهواز، اهواز، ایران
4 دانشجوی دکتری گروه فیزیولوژی ورزشی، دانشکده علوم ورزشی ،دانشگاه شهید چمران اهواز، اهواز، ایران
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
Background and Purpose: Nonalcoholic fatty liver disease (NAFLD) is one of the most common metabolic disorders in today's societies, which occurs due to excessive accumulation of fat in the liver tissue without alcohol consumption and can be accompanied by inflammation, nonalcoholic steatohepatitis, fibrosis, and ultimately liver cirrhosis. One of the important pathways involved in the progression of this disease is the disruption of the regulation and synthesis of bile acids, as well as changes in the expression of hepatic transporters responsible for the absorption, excretion, and transport of these acids. Transporters such as Bsep, Ntcp, and Oatp play a fundamental role in bile acid homeostasis, and their inappropriate decrease or increase can play a role in bile acid accumulation, increased inflammation, and the progression of NAFLD. On the other hand, scientific evidence shows that lifestyle interventions, especially continuous exercise and the use of anti-inflammatory supplements such as omega-3, can have significant effects on improving liver function and regulating metabolic pathways. However, the simultaneous effect of these two interventions on the expression of bile acid transporters and the amount of bile acids in liver tissue has not yet been investigated in detail.
Materials and Methods: In this study, 30 adult male Wistar rats were randomly divided into five following groups: healthy control, NAFLD, NAFLD + MICT, NAFLD + OM3, NAFLD + MICT + OM3. Rats were fed with high fat diet for 12 weeks. All animals received daily 25% sucrose in drinking water for 12 weeks. The training protocol consisted of four sessions per week for 8 weeks with a speed of 18–22 m/min for 50 minutes per session. The hepatic expression of bile acids transporters (NTCP and BESB) proteins was measured by Western blot method. The concentrations of colic acid (CA) and chenodeoxycholic acid (CDCA) were measured by ElISA method. Blood glucose levels and lipid profile were assessed using an autoanalyzer.
Results: The findings showed that both continuous exercise intervention and omega-3 consumption independently significantly increased the expression of bile acid transporters and reduced the accumulation of bile acids in the liver tissue. (P<0.05) These effects included a significant improvement in the expression of Bsep (responsible for bile excretion into the canaliculus), an increase in Ntcp (a carrier of bile entry into the hepatocyte), and proper regulation of Oatp. In the combined group, these changes were more intense, such that the simultaneous exercise and omega-3 caused a synergistic effect and the highest level of increased expression of transporters and the highest reduction in bile acids were observed. Also, the apparent improvement of liver tissue in the combined group was more evident than in the other groups. (P<0.05)
Conclusion: Our findings showed that both exercise training intervention and omega-3 consumption independently improved liver function by regulating the expression of bile acid transporters in the liver of NAFLD mice. Furthermore, the combination of exercise training and omega-3 consumption had a synergistic effect, providing a greater effect on maintaining bile acid homeostasis compared to either intervention alone. These results support the possibility of combining exercise training and omega-3 fatty acid consumption as an effective method for managing NAFLD and improving metabolic health in patients with NAFLD.
کلیدواژهها [English]
)