اثر هشت هفته تمرین استقامتی بر کلاژن 18 پلاسمایی و عضله قلبی موشهای صحرایی نر ویستار

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشگاه آزاد اسلامی

2 دانشگاه شهید بهشتی

چکیده

چکیدههدف: هدف از این پژوهش بررسی اثر یک دوره تمرین استقامتی بر کلاژن 18 پلاسمایی و عضله قلبی موشهای نر ویستار بود.و تمرین (C) 135 گرم) به طور تصادفی به دو گروه کنترل - 6 هفتهای و 125 - روش شناسی: 16 سر موش صحرایی نر ویستار ( 4تقسیم شدند. برنامه تمرینی شامل 8 هفته، 5 روز در هفته، به مدت 20 تا 60 دقیقه و سرعت 28 تا 34 متر در دقیقه بود. (T)72 ساعت پس از آخرین جلسه تمرینی نمونهگیری خون و بافت برداری انجام شد. مقدار کلاژن 18 به روش الایزا اندازهگیری شد.تجزیه و تحلیل شد. نتایج: مقدار کلاژن 18 پلاسمایی α<0/ مستقل در سطح معنیداری 05 t دادهها با استفاده ازپس از هشت هفته فعالیت (T=40/476±11/933 ،C=20/080±3/ و عضله قلبی ( 169 (T=0/839±0/079 ،C=0/721±0/142)بحث و نتیجهگیری: افزایش کلاژن .( P=0/ ورزشی افزایش داشت، ولی این افزایش فقط در سطح عضله قلبی معنیدار بود ( 04618 احتمالاً نشان دهنده تأثیر فعالیتهای ورزشی بر مهار رگزایی بوده و گواه دیگری بر اثرات ضد سرطانی فعالیتهای ورزشیمیباشد.

کلیدواژه‌ها


منابع
- O'Reilly MS, Holmgren L, Shing Y, Chen C,
Rosenthal RA, Moses M, et al. (1994).
Angiostatin: a novel angiogenesis inhibitor that
mediates the suppression of metastases by a
Lewis lung carcinoma. cell. 79(2):315-28.
- Hanahan D.( 1996). Patterns and emerging
mechanisms of the angiogenic switch during
tumorigenesis. cell.86:353-64.
- Folkman J, Merler E, Abernathy C, Williams
G.( 1971). Isolation of a tumor factor
responsible for angiogenesis. The Journal of
experimental medicine.133(2):275-88.
- Richardson R, Wagner H, Mudaliar S, Saucedo
E, Henry R, Wagner P.( 2000). Exercise
adaptation attenuates VEGF gene expression in
human skeletal muscle. American Journal of
اثر هشت هفته تمرین استقامتی بر کلاژن 18 پلاسمایی و عضله قلبی موشهای صحرایی نر ویستار 700
Physiology-Heart and Circulatory Physiology
.279(2):H772-H8.
- Asano M, Kaneoka K, Nomura T, Asano K,
Sone H, Tsurumaru K, et al.( 1998). Increase in
serum vascular endothelial growth factor levels
during altitude training. Acta physiologica
scandinavica.162(4):455-60.
- Gunga HC, Kirsch K, Röcker L, Behn C,
Koralewski E, Davila EH, et al.( 1999).
Vascular endothelial growth factor in exercising
humans under different environmental
conditions. European journal of applied
physiology and occupational
physiology.79(6):484-90.
- Gustafsson T, Knutsson A, Puntschart A,
Kaijser L, Nordqvist SAC, Sundberg C ,et al.
Increased expression of vascular endothelial
growth factor in human skeletal muscle in
response to short-term one-legged exercise
training. Pflügers Archiv European Journal of
Physiology.444(6):752-9.
- Saarela J, Rehn M, Oikarinen A, Autio-
Harmainen H, Pihlajaniemi T.( 2002). The short
and long forms of type XVIII collagen show
clear tissue specificities in their expression and
location in basement membrane zones in
humans. The American journal of pathology.
;153(2):611-26.
- Sasaki T, Fukai N, Mann K, Göhring W, Olsen
BR, Timpl R.( 1998). Structure, function and
tissue forms of the C-terminal globular domain
of collagen XVIII containing the angiogenesis
inhibitor endostatin. The EMBO
journal.17(15):4249-56.
- O'Reilly MS, Boehm T, Shing Y, Fukai N,
Vasios G, Lane WS, et al.( 1997). Endostatin:
an endogenous inhibitor of angiogenesis and
tumor growth. cell.88(2):277-85.
- Marneros AG, Olsen BR.( 2005). Physiological
role of collagen XVIII and endostatin. The
FASEB Journal.19(7):716-28.
- Ferreras M, Felbor U, Lenhard T, Olsen BR,
Delaisse JM.( 2000). Generation and
degradation of human endostatin proteins by
various proteinases. FEBS letters.486(3):247-
- Kovanen V, Suominen H.( 1989). Age-and
training-related changes in the collagen
metabolism of rat skeletal muscle. European
journal of applied physiology and occupational
physiology.58(7):765-71.
- Thomas DP, McCormick RJ, Zimmerman SD,
Vadlamudi RK, Gosselin L.( 1992). Aging-and
training-induced alterations in collagen
characteristics of rat left ventricle and papillary
muscle. American Journal of Physiology-Heart
and Circulatory Physiology.263(3):H778-H83.
- Marneros AG.( 2009). Tumor angiogenesis in
melanoma. Hematology/oncology clinics of
North America.23(3):431.
- Kivela R, Silvennoinen M, Lehti M, Jalava S,
Vihko V, Kainulainen H.( 2008). Exerciseinduced
expression of angiogenic growth
factors in skeletal muscle and in capillaries of
healthy and diabetic mice. Cardiovasc Diabetol.
(13):10.1186.
- Deininger MH, Fimmen B, Kremsner PG ,
Meyermann R, Schluesener HJ.( 2002).
Accumulation of endostatin/collagenXVIII in
brains of patients who died with cerebral
malaria. Journal of
neuroimmunology.131(1):216-21.
- Mueller C, Schluesener H, Fauser U, Conrad S,
Schwab J.( 2007). Lesional expression of the
endogenous angiogenesis inhibitor
endostatin/collagen XVIII following traumatic
brain injury (TBI). Experimental neurology.
(2):228-37.
- Castro-Giner F, Bustamante M, Gonzalez JR,
Kogevinas M, Jarvis D, Heinrich J, et al.(
. A pooling-based genome-wide analysis
identifies new potential candidate genes for
atopy in the European Community Respiratory
Health Survey (ECRHS). BMC medical
genetics.10(1):128.
- Santos S, Oliveira G, Tavares A, Massensini A,
Carvalhaes L, Reljasvaara R, et al.( 2005).
Collagen XVIII and fibronectin involvement in
bullous scleroderma. Dermatology online
journal.11(1):17.
- Musso O, Theret N, Heljasvaara R, Rehn M,
Turlin B, Campion JP, et al.( 2003). Tumor
hepatocytes and basement membrane–
Producing cells specifically express two
different forms of the endostatin precursor,
collagen XVIII, in human liver cancers.
Hepatology.33(4):868-76.
- Hu TH, Huang CC, Wu CL, Lin PR, Liu SY,
Lin JW, et al.( 2004). Increased
endostatin/collagen XVIII expression correlates
with elevated VEGF level and poor prognosis in
hepatocellular carcinoma. Modern pathology.
(5):663-72.
- Passos-Bueno MR, Suzuki OT, Armelin-Correa
LM, Sertie AL, Errera IV F, Bagatini K, et al.(
. Mutations in Collagen 18A1 (COL18A1)
and their relevance to the human phenotype.
Anais da Academia Brasileira de Ciências.
(1):123-31.
- Fathi R, Ghanbari-Niaki A, Rahbarizadeh F,
Hedayati M, Ghahramanloo E, Farshidi Z.(
حجت الله نیکبخت و همکاران
. The effect of exercise on plasma acylated
ghrelin concentrations and gastrocnemius
muscle MRNA expression in male rats. Iranian
Journal of Endocrinology and Metabolism.
(5):519-26.
- van Royen N, Piek JJ, Schaper W, Bode C,
Buschmann I.( 2001). Arteriogenesis:
mechanisms and modulation of collateral artery
development. Journal of Nuclear
Cardiology.8(6):687-93.
- Risau W.( 1997). Mechanisms of angiogenesis.
Nature.386(6626):671-4.
- Muragaki Y, Abe N, Ninomiya Y, Olsen BR,
Ooshima A.( 1994). The human alpha 1 (XV)
collagen chain contains a large amino-terminal
non-triple helical domain with a tandem repeat
structure and homology to alpha 1 (XVIII)
collagen. Journal of Biological
Chemistry.269(6):4042-6.
- Rehn M, Pihlajaniemi T.( 1994). Alpha 1
(XVIII), a collagen chain with frequent
interruptions in the collagenous sequence, a
distinct tissue distribution, and homology with
type XV collagen. Proceedings of the National
Academy of Sciences. 91(10):4234-8.
- Väänänen A, Ylipalosaari M, Parikka M,
Kainulainen T, Rehn M, Heljasvaara R, et al.(
. Collagen XVIII modulation is altered
during progression of oral dysplasia and
carcinoma. Journal of oral pathology &
medicine. 36(1):35-42.
- Quelard D, Lavergne E, Hendaoui I, Elamaa H,
Tiirola U, Heljasvaara R, et al.( 2008). A
Cryptic Frizzled Module in Cell Surface
Collagen 18 Inhibits Wnt/β −Catenin Signaling.
PLoS One.3(4):e1878.
- Sakimoto T, Kim T-i, Ellenberg D, Fukai N,
Jain S, Azar DT, et al.( 2008). Collagen XVIII
and corneal reinnervation following
keratectomy. FEBS letters. 582(25):3674-80.
- Muragaki Y, Timmons S, Griffith CM, Oh SP,
Fadel B, Quertermous T, et al.( 1995). Mouse
Col18a1 is expressed in a tissue-specific manner
as three alternative variants and is localized in
basement membrane zones. Proceedings of the
National Academy of Sciences.92(19):8763-7.
- Ylikärppä R ,Eklund L, Sormunen R, Kontiola
AI, Utriainen A, Määttä M, et al.( 2003). Lack
of type XVIII collagen results in anterior ocular
defects. The FASEB Journal. 17(15):2257-9.
- Määttä M, Heljasvaara R, Pihlajaniemi T,
Uusitalo M.( 2007). Collagen XVIII/endostatin
shows a ubiquitous distribution in human ocular
tissues and endostatin-containing fragments
accumulate in ocular fluid samples. Graefe's
Archive for Clinical and Experimental
Ophthalmology. 245(1):74-81.
- Musso O, Rehn M, Saarela J, Theret N, Lietard
J, Hintikka E, et al.( 1998). Collagen XVIII is
localized in sinusoids and basement membrane
zones and expressed by hepatocytes and
activated stellate cells in fibrotic human liver.
Hepatology.28(1):98-107.
- Beck L, D'Amore PA.( 1997). Vascular
development: cellular and molecular regulation.
The FASEB journal.11(5):365-73.
- Celletti FL, Hilfiker PR, Ghafouri P, Dake
MD.( 2001). Effect of human recombinant
vascular endothelial growth factor165 on
progression of atherosclerotic plaque. Journal of
the American College of Cardiology.
(8):2126-30.
- Hamano Y, Okude T, Shirai R, Sato I, Kimura
R, Ogawa M, et al.( 2010). Lack of collagen
XVIII/endostatin exacerbates immune-mediated
glomerulonephritis. Journal of the American
Society of Nephrology. 21(9):1445-5.5
- Lin Y, Zhang S, Rehn M, Itaranta P, Tuukkanen
J, Heljasvaara R, et al.(2000) Induced
repatterning of type XVIII collagen expression
in ureter