تاثیر شناوری در آب سرد پس از فعالیت ورزشی اسنتریک پاسخ های رشدی، التهابی و آسیب عضلانی عضله اسکلتیFHL موش های صحرایی

نوع مقاله : علمی - پژوهشی

نویسندگان

1 داﻧﺸﮕﺎه ﺗﻬﺮان

2 ﻋﻠﻮم ﭘﺰﺷﮑﯽ ﺷﻬﯿﺪ ﺑﻬﺸتی

چکیده

هدف:شناوری در آب سرد برای سرعت بخشیدن به بازیافت پس از تمرینات شدید و آسیب زا مورد توجه ورزشکاران قرارگرفته است. با توجه به اهمیت سلول های ماهواره ای و نقش پاسخ های التهابی در روند ترمیم، هدف از این پژوهش بررسی تاثیر شناوری در آب سرد پس از فعالیت ورزشی اسنتریک بر بیان ژن MyoD به عنوان شاخص فعال سازی سلول های ماهواره ای ،CK به عنوان شاخص غیر مستقیم آسیب و  IL-6 و IL-10 بعنوان شاخص های التهابی در موش های نر بود. روش شناسی: 30 سر موش جوان سالم ونژاد ویستار (دامنه وزنی 10±300 گرم) به طور تصادفی درپنج گروه شش تایی به عنوان نمونه انتخاب شدند. طرح تحقیق تجربی بود و آزمودنی ها پروتکل فعالیت ورزشی اسنتریک به مدت 90 دقیقه را اجرا کردند. دو گروه دروهله های زمانی 3 و 48 ساعت بازیافت از فعالیت به همراه 10 دقیقه شناوری در آّب سرد و دو گروه با همان دوره های زمانی بازیافت بدون  شناوری در آب سرد و یک گروه به عنوان کنترل بررسی شدند. تغییرات بیان ژن MyoD در عضله اسکلتی FHL و سطوح سرمی CK، IL-6 و IL-10در چهار گروه مقایسه شد. داده های بیان ژن با نرم افزار Rest و داده های سرمی با استفاده از آزمون t مستقل تجزیه و تحلیل شد. نتایج: نتایج نشان داد mRNA MyoD در گروه سه ساعت پس از فعالیت ورزشی اسنتریک همراه با شناوری در آب سرد افزایش یافت و سطوح IL-6 و IL-10 در این گروه نسبت به گروه سه ساعت بعداز فعالیت اسنتریک بدون شناوری در آب سرد کاهش معنی دار داشتند ولی تغییرات CK معنی دار نبود.تغییر معنی داری در mRNA MyoD و Ck, IL-6, IL-10 سرمی در 48 ساعت پس از فعالیت اسنتریک همراه با شناوری مشاهده نشد.  نتیجه گیری: بر اساس یافته های این تحقیق احتمالا  استفاده از آب سرد در زمان بازیافت موجب فعال سازی سلول های ماهواره ای و کاهش پاسخ های التهابی درفاز اولیه آسیب پس از فعالیت ورزشی اسنتریک می شود.

کلیدواژه‌ها

عنوان مقاله [English]

The effect of cold water immersion after eccentric exercise on myogenic, inflammatory and muscle damage responses In FHL skeletal muscle in rats

چکیده [English]



Purpose: cold water immersion is considered to accelerate the recovery from damaging exercise for athletes. Given
the importance of satellite cells and the role of inflammatory responses in regeneration process, the purpose of this
study was to investigate the effect of cold water immersion on MyoD gene expression as an activation marker of
satellite cells, CK as an indirect marker of damage and IL-6, IL-10 as inflammatory markers after eccentric exercise
in male rats. Methods: 30 young and healthy male Wistar rats (Weight range= 300±10) were assigned randomly in
5 groups each consisting of 6 subjects. It was an experimental research and subjects participated in eccentric
exercise protocol (90min). Then they were compared in tow groups with and without cold water immersion
(10min), in 3 and 48 hours after exercise in changes of MyoD gene expression in FHL skeletal muscle and the level
of serum CK,IL-6 and IL-10. We used Rest software for analyzing MyoD gene expression and independent T test
for analyzing serum data's. Results: Results showed that mRNA MyoD has been increased three hours after
eccentric exercise in cold water immersion group and the level of IL-6, IL-10 decreased significantly in that group
in compare with the group of three hours after eccentric exercise without cold water immersion. There is no
significant change in CK. There was no significant changes in mRNA MyoD and serum CK,IL-6,IL-10 in 48 hour
after eccentric exercise and cold water immersion. Conclusion: Based on findings of the present study, cold water
during recovery may cause the activation of satellite cells and decrease the inflammatory responses after eccentric
exercise in early phase of damage.

کلیدواژه‌ها [English]

  • damaging exercise
  • satellite cells
  • cold water immersion
  • inflammation

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سابقه مقاله

  • تاریخ دریافت: 18 آذر 1394
  • تاریخ بازنگری: 23 خرداد 1403
  • تاریخ پذیرش: 11 دی 1399
  • تاریخ اولین انتشار: 11 دی 1399
  • تاریخ انتشار: 01 اسفند 1393

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