اثرتمرینات فزاینده شمشیربازی بر بیان ژن و فعالیت سرمی ماتریکس متالوپروتئیناز 2 (MMP2) در دختران شمشیرباز نخبه

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشیار گروه آسیب شناسی و حرکات اصلاحی دانشگاه علامه طباطبائی تهران ( هسته پژوهشی فیزیولوژی تندرستی و فعالیت بدنی)

2 کارشناسی ارشد فیزیولوژی ورزشی دانشگاه ارومیه

3 دانشجوی دکتری تخصصی گرایش فیزیولوژی ورزشی قلب و عروق و تنفس دانشگاه ارومیه

چکیده

هدف تحقیق: متالوپروتئینازهای ماتریکس (MMPs) نقش مهمی در هموستاز ماتریکس خارج سلولی عضلات اسکلتی بازی می‌کنند. هدف از پژوهش حاضر بررسی اثر هشت هفته تمرینات شمشیربازی فزاینده بر بیان ژن و فعالیت سرمی ماتریکس متالوپروتئیناز2 دختران شمشیرباز نخبه بود. روش تحقیق: در این پژوهش 19 شمشیرباز دختر جوان داوطلبانه انتخاب و به صورت تصادفی در دو گروه تجربی و کنترل تقسیم‌بندی شدند. آزمودنی‌های گروه تجربی 8 هفته و هر هفته 5 جلسه در تمرین شمشیربازی فزاینده شرکت کردند. در حالی که گروه کنترل در این مدت هیچ گونه برنامة تمرینی نداشتند. نمونه‌های خونی ناشتای آزمودنی قبل، 4 هفته بعد و نهایتا 48 ساعت پس از اتمام دوره تمرینی گرفته شد. بیان ژن MMP2 آزمودنی‌ها با استفاده از روش Real time-PCR اندازه‌گیری شد و فعالیت MMP2 به روش الایزا اندازه‌گیری گردید. در پایان با استفاده از آزمون آماری t مستقل میانگین بیان ژن mmp2 دو گروه تجربی و کنترل مقایسه شد. نتایج: نتایج این پژوهش نشان داد که میزان بیان mRNA ژن MMP2 پس از 8 هفته تمرین در گروه تجربی در مقایسه با گروه کنترل کاهش معناداری داشت (05/0P<). بحث و نتیجه‌گیری: فعالیت پروتئینازی MMP2 و بیان ژن این آنزیم پس از تمرینات فزاینده شمشیربازی در افراد فعال کاهش می‌یابد که در نتیجه، فعالیت بدنی سبب کاهش تخریب ماتریکس خارج سلولی و صدمات بافت عضلانی در دختران شمشیرباز و فعال می‌شود. 

کلیدواژه‌ها

عنوان مقاله [English]

Effect of progressive fencing exercis training on gene expression and serum activity of matrix metalloproteinase 2 (MMP2) in elite girls fencer

چکیده [English]



Purpose: Matrix metalloproteinases (MMPs) play an important role in the homeostasis of the extracellular matrix (ECM)
in skeletal muscle. The purpose of the current study was to investigate the effect of 8 weeks progressive fencing exercises
on MMP2 activity and gene expression in elite fencer girls. Method: In this study, 19 young athlete girls were voluntarily
selected and randomly allocated to the experimental and control groups. Subjects in the experimental group performed
five sessions of progressive fencing exercise per week for 8 weeks, while the control group didn't participate in any
exercise programs. A Fasting blood sample was taken from all participants before, after four weeks and finally 48 hours
after the exercise program. MMP2 gene expression was measured using Real time-PCR and Serum MMP2 activity was
measured by ELISA method. Finally, to compare the mean of mmp2 gene expression in experimental and control group,
independent t-test was used. Results: Results showed that expression of MMP2 mRNA was significantly decreased
following eight week progressive fencing exercises in the exercise group in comparison with the control group (P<0.05).
Conclusions: Proteinase activity of MMP2 and gene expression of this enzyme is reduced in active individuals after
progressive fencing training; in conclusion physical activity reduces the degradation of extracellular matrix and muscle
tissue injury in elite fencer girls.

کلیدواژه‌ها [English]

  • Gelatinase A gene
  • progressive fencing exercises
  • athlete girls

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سابقه مقاله

  • تاریخ دریافت: 14 اسفند 1395
  • تاریخ بازنگری: 22 خرداد 1403
  • تاریخ پذیرش: 11 دی 1399
  • تاریخ اولین انتشار: 11 دی 1399
  • تاریخ انتشار: 01 آذر 1395

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