تاثیر تمرین هوازی و هوای آلوده به ذرات کربن سیاه بر بیان ژنهای NF-κB و TNF-α بافت هیپوکامپ مغز موشهای صحرایی نر

نویسندگان

1 گروه تربیت بدنی و علوم ورزشی، دانشگاه تربیت مدرس، تهران، ایران

2 گروه علوم ورزشی و تندرستی، دانشگاه شهید بهشتی، تهران، ایران

3 دانشگاه زنجان، زنجان، ایران

چکیده

هدف: هدف از این پژوهش بررسی تاثیر تمرین هوازی و هوای آلوده به ذرات کربن سیاه بر بیان ژنهای NF-κB و TNF-α هیپوکامپمغز موشهای صحرایی نر بود.روشها: بیست و چهار موش صحرایی نر ویستار )با میانگین توده بدنی 97 / 26 ± 29 / 279 گرم( بهطور تصادفی در چهار گروه: الف-بدون تمرین و بدون قرار گرفتن در معرض هوای آلوده به ذرات کربن سیاه؛ ب- تمرین هوازی؛ ج- قرار گرفتن در معرض ذرات؛ ود- تمرین و با قرار گرفتن در معرض ذرات کربن سیاه قرار گرفتند. برای قرار دادن حیوانات در معرض ذرات کربن سیاه با قطرکمتر از 10 میکرون از دستگاه تزریق ذرات و اتاقک استفاده شد. مدت تمرین هوازی با 50 درصد سرعت بیشینه هر گروه 4 هفتهبود و 24 ساعت پس از آخرین جلسه تمرینی و قرارگیری در معرض ذرات کربن سیاه حیوانات قربانی شدند. با استفاده از روشReal time-Pcr بیان ژن NF-κB و TNF-α در بافت هیپوکامپ موشها ارزیابی شد. برای تعیین معنادار بودن تفاوت بین گروههااز تحلیل واریانس دوطرفه و آزمون تعقیبی LSD استفاده شد.نتایج: تفاوت معناداری بین گروههای الف و ب ) 001 / 0 = P (، الف و د ) 003 / 0 = P ( در مقدار TNF-α وجود داشت. تفاوت معنادارینیز بین گروههای الف و ب ) 008 / 0 = P( در مقدار NF-κB دیده شد.نتیجهگیری: چهار هفته تمرین هوازی و هوای آلوده به ذرات کربن سیاه باعث تعدیل عوامل التهابی NF-κB و TNF-α میشود. بهنظر میرسد این تغییرات در بخشی با کاهش خطر ابتلا به بیماریهای عصبی مغزی همراه باشد.واژههای کلیدی: تمرین هوازی، ذرات معلق، التهاب، هیپوکامپ.

عنوان مقاله [English]

تاثیر تمرین هوازی و هوای آلوده به ذرات کربن سیاه بر بیان ژنهای NF-κB و TNF-α بافت هیپوکامپ مغز موشهای صحرایی نر

نویسندگان [English]

  • بتول رضایی سراجی 1
  • حمید آقا علی نژاد 1
  • رضا قرخانلو 1
  • معصومه ابتکار 1
  • محمد فشی 2
  • بهزاد پاکراد 1
  • اکرم کریمی اصل 3
1 گروه تربیت بدنی و علوم ورزشی، دانشگاه تربیت مدرس، تهران، ایران
2 گروه علوم ورزشی و تندرستی، دانشگاه شهید بهشتی، تهران، ایران
3 دانشگاه زنجان، زنجان، ایران
چکیده [English]

Introduction: The aim of this study was to examine the effect of aerobic exercise and carbon particles on gene expression of NF-κB and TNF-α in rats. Materials and Methods: 24 male Wistar rats with an average body mass 279.29±26.97 g were randomly divided into 4 groups (A) Control (without training and without exposure to black carbon particles ), (b)control-pollution (exposure to black carbon particles), (c) exercise (aerobic exercise), (d) pollution-practice ( aerobic exercise with exposure to black carbon) groups.particles with a diameter less than 10 micron was used. Aerobic training with 50 percent of Max speed for 4 weeks. 24 hours after the last training session and exposure, the animals were sacrificed. Real time-Pcr technique was used. Two-way ANOVA and post hoc LSD was used to determine the significance of differences between groups. Results:there was significant difference between groups a and b (p=0.001) and b and d (p=0.003) in the amount of TNF-α. significant difference between groups (p=0.008) in the amount of NF-κB was observed. Conclusion: 4 weeks of aerobic exercise and polluted air lead to dampen the inflammatory factors NF-κB and TNF-α. . It seems that these changes lead to reducing the risk of neurodegenerative diseases.

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