نوع مقاله : علمی - پژوهشی

نویسندگان

دانشکده تربیت‌بدنی و علوم ورزشی، دانشگاه تهران، تهران، ایران

چکیده

هدف: فرایند عضله­ زایی نقش مهمی در بازسازی و رشد عضلۀ اسکلتی دارد. با وجود این،  تأثیر تمرین­ های تناوبی خیلی شدید (HIIT) بر عوامل این فرایند مشخص نیست. هدف از پژوهش حاضر، بررسی تأثیر هشت هفته HIIT بر عوامل مهم عضله ­زایی ـ عامل عضله ­زایی 5 (MyF5) و میوژنین ـ در عضلۀ دوقلوی موش­ های صحرایی بود.روش­ شناسی: در این مطالعه  تجربی، 16 سر موش صحرایی نر نژاد ویستار با سن 10 هفته و محدوده وزنی 25 ± 225 گرم، تصادفی به دو گروه کنترل و HIIT تقسیم شدند. برنامه تمرین HIIT به مدت 40 دقیقه، سه روز در هفته و به مدت هشت هفته اجرا شد. 48 ساعت پس از آخرین جلسه تمرینی، عضله دو­قلو استخراج و میزان بیان ژن MyF5 و میوژنین به روش Real time-PCR سنجیده شد. برای تجزیه و تحلیل داده ­های آماری از آزمون t مستقل استفاده شد.نتایج: بیان ژن MyF5 و میوژنین در گروه HIIT در مقایسه با گروه کنترل به صورت معناداری افزایش داشت. همچنین، وزن عضلۀ دوقلو در گروه تمرینی در مقایسه با گروه کنترل افزایش معناداری داشت.بحث و نتیجه ­گیری: HIIT، به افزایش بارز بیان ژن MyF5، میوژنین و وزن عضلۀ دوقلوی موش ­های صحرایی منجر شد. 

کلیدواژه‌ها

عنوان مقاله [English]

The effect of a period of High-intensity Interval Training on myogenesis factors gene expression in the skeletal muscle of rats

نویسندگان [English]

  • Soheil Biglari
  • Abbas Ali Gaeini

the Department of Sport Physiology, Faculty of Physical Education and Sports Sciences, University of Tehran, Tehran, Iran

چکیده [English]

Purpose: Myogenesis plays an important role in the skeletal muscle reconstruction and growth. However, the impact of High-intensity Interval Training (HIIT) on the factors of this process is unknown. The purpose of this study was to investigate the effect of 8 weeks HIIT on myogenesis main factors –(Myogenic Factor 5) Myf5 and Myogenin- in the rat's gastrocnemius muscle.
Methods: In this experimental study, sixteen male Wistar rats (weight: 255 ± 25 g) were randomly divided into two groups, control (n = 8) and HIIT (n = 8). HIIT protocol was performed for 40 minutes, three days a week for eight weeks. 48 hours after the last training session, gastrocnemius muscle was extracted and MyF5 and myogenin gene expression were measured by Real Time-PCR. To analyze the data, independent T-test was used.
Results: MyF5 and myogenin gene expression significantly increased in the experimental group compared to the control group (P≤0.05). Additionally, gastrocnemius muscle weight in training group was significantly higher than the control group (P≤0.01).
Conclusions: HIIT resulted in a significant increase in the expression of MyF5, Myogenin, and gastrocnemius muscle weight in rats.

کلیدواژه‌ها [English]

  • HIIT- myogenesis- MyF5- Myogenin- Real time-PCR
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