تأثیر تمرین تناوبی با شدت بالا بر بیان ژن شاخص‌های آپوپتوز در بافت قلب موش‌های نر پیر نژاد C57BL/6

نوع مقاله : علمی - پژوهشی

نویسندگان

دانشگاه تهران

چکیده

سابقه و هدف: ژن‌های Bax و Bcl-2 از جمله ژن‌های کلیدی مسیر داخلی آپوپتوز می‌باشند. آپوپتوز در بافت قلب در دوران پیری اتفاق می‌افتد. هدف از پژوهش حاضر، تعیین تأثیر تمرین تناوبی با شدت بالا بر بیان ژن شاخص‌های آپوپتوز قلب (Bax  و Bcl2) در بافت قلب موش‌های نر پیر بود. روش تحقیق: این مطالعه تجربی روی 20 سر موش نر 24 ماهه نژاد C57BL/6 انجام شد. حیوانات بطور تصادفی به دو گروه کنترل (10 سر) و آزمایش (10 سر) تقسیم شدند. گروه آزمایش به مدت 4 هفته و هر هفته 5 جلسه به تمرین تناوبی پرشدت پرداختند. در ابتدا تمرین تناوبی با شدتی برابر با 85 درصد حداکثر سرعت شروع و در ادامه برنامه تناوبی، هفته‌ای پنج درصد به ‌سرعت افزوده شد و دو هفته آخر با 95 درصد سرعت خاتمه یافت. 48 ساعت پس از آخرین جلسه‌ی تمرین، حیوانات قربانی و بافت قلبی جدا شد. بیان ژن‌های BCL-2 و Bax به وسیله SYBR-Green Real-time PCR مورد بررسی قرار گرفت. نتایج تحقیق: نتایج حاصل از Real Time-PCR نشان داد که در گروه آزمایش بیان ژن پروآپوپتوتیک Bax در مقایسه با گروه کنترل (به ترتیب 022/0±618/0 و1) کاهش پیدا کرده‌است (05/0p<). هم‌چنین میزان بیان ژن آنتی‌آپوپتوتیک Bcl2 در گروه تجربی در مقایسه با گروه کنترل (084/0±824/1و 1) افزایش یافت (05/0p<). بحث و نتیجه‌گیری: نتایج این مطالعه نشان داد که تمرین تناوبی با شدت بالا با کاهش بیان Bax و افزایش بیان Bcl-2 در کاهش آپوپتوز در بافت قلبی موش‌های پیر مؤثر می‌باشد. 

کلیدواژه‌ها


عنوان مقاله [English]

Effect of high-intensity interval training on expression of apoptotic markers in cardiac tissue of aged male C57BL/6 mice

نویسندگان [English]

  • Rahman Soori
  • Maryam Zare Shahneh
  • Sirus Choubineh
  • Azam Ramezankhani
Tehran university
چکیده [English]

Purpose: Bax and BCL-2 genes are key molecules that involve in intrinsic pathway of apoptosis. Apoptosis in cardiac tissue occurs during aging. The aim of the present study was to investigate the effect of high-intensity interval training on expression of cardiac apoptotic markers (Bax and BCL-2) in aged male mice. Materials & Methods: this experimental study was performed on twenty 24- months old C57BL/6 mice. Animals were randomly assigned to the control (n=10) and experimental groups (n=10). The experimental group performed high interval intensity training for four weeks (five sessions per week). At first the interval training started at the intensity equal to 85 percent of maximum speed and in the continuation of interval training, 5 percent was added weekly and the last two weeks ended with 95 percent rate. Forty-eight hours after the last training session, animals were anesthetized, myocardial muscle was removed. Bax and BCL-2 genes expression were investigated by SYBR-Green Real-time PCR analysis. Results: The results of Real Time-PCR showed that the expression of pro-apoptotic Bax gene in experimental group was decreased compared to control group (0.618±0.022, 1) (p< 0.05). Also, Anti-apoptotic Bcl2 gene expression in the experimental group was increased compared to the control group (1.824±0.084, 1) (p< 0.05). Conclusions: The results of this study showed that high-intensity interval training by reducing the expression of Bax and increasing Bcl-2 expression may be effective in reducing apoptosis in cardiac tissue of aged mice.

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  • BCL-2- Bax- high-intensity interval training- cardiac apoptosis
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