تاثیر فعالیت ورزشی با شدت و حجم متفاوت بر بیان ژن FGF2 در بافت چربی احشایی و زیر پوستی در رت های نر

نوع مقاله : علمی - پژوهشی

نویسندگان

1 استادیار، گروه تربیت بدنی و علوم ورزشی، دانشکده علوم انسانی، دانشگاه شهرکرد، شهرکرد، ایران

2 کارشناسی ارشد فیزیولوژی ورزشی، دانشگاه پیام نور، ایران

3 دانشیار، فیزیولوژی ورزشی، دانشگاه پیام نور، ایران

چکیده

هدف: هدف مطالعه حاضر بررسی تأثیر دو شیوه تمرین تناوبی شدید(HIIT) و تمرین تداومی با شدت متوسط(MICT) بر بیان ژن FGF2 در بافت چربی احشایی و زیرپوستی در رت­های نر نژاد ویستار بود.روش شناسی: روش مطالعه حاضر از نوع تجربی بود. نمونه آماری شامل 24 سر رت نر ویستار بودند که به گروه­های اصلی کنترل پایه، کنترل 8 هفته، تمرین HIIT و تمرین MICT تقسیم شدند. گروه­های تمرین ورزشی به مدت 8 هفته و هر هفته 5 جلسه تمرین دویدن بر روی تردمیل جوندگان انجام دادند. رت­های گروه MICT به مدت 15 تا 60 دقیقه و با شدت 50 تا 75% حداکثر اکسیژن مصرفی تمرین کردند و در گروه HIIT 4 تا8 تناوب یک دقیقه­ای با شدت 70 تا100% حداکثر اکسیژن مصرفی و تناوب­های استراحت فعال با  شدت 50 تا %70 حداکثر اکسیژن مصرفی اجرا شد. بافت چربی احشایی و زیرپوستی 48 ساعت پس از آخرین جلسه تمرین برداشته شد و سنجش بیان ژن  FGF2با روش Real time PCR انجام شد.نتایج: نتایج نشان داد هر دو نوع مداخله­ی ورزشی سبب افزایش غیر معنی­دار بیان ژن FGF-2 در بافت چربی زیر­پوستی شدند(05/0P≥). برخلاف این، در بافت چربی احشایی بیان این ژن به دنبال هر دو مداخله­ی ورزشی به طور غیر معنی­داری کاهش داشت(05/0P≥).بحث و نتیجه گیری: بر اساس یافته­های تحقیق به نظر می­رسد دو نوع بافت چربی زیر­پوستی و احشایی پاسخ متفاوتی به فعالیت ورزشی می­دهند و احتمالاً این دو بافت به لحاظ آنژیوژنیکی فعالیت متفاوتی دارند، هر چند عوامل آنژیوژنیکی مختلف و متعددی در این فرآیند مشارکت دارند که احتیاج به مطالعه بیشتری است.

عنوان مقاله [English]

The Effect of Exercise with Different Intensity and Volume on FGF2 Gene Expression in Subcutaneous and Visceral Adipose tissue in Male Rats

نویسندگان [English]

  • Ghamar Mirzaei Amirabadi 1
  • Mohammad Reza Asad 2
  • Mostafa Rahimi 3
1 Graduated student of Exercise Physiology, University of Payam Noor, Iran
2 Associate professor, Exercise Physiology, University of Payam Noor, Iran
3 Assistant professor, Department of Physical Education and Sport Sciences, Faculty of Humanities, Shahrekord University, Shahrekord, Iran.
چکیده [English]

Purpose: The purpose of present research was to study the effects of high intensity interval training (HIIT) and moderate intensity continues training (MICT (on FGF2 gene expression in visceral and subcutaneous adipose tissues in male Wistar rat.
Methods: In this experimental study, twenty four male Wistar rat as study sample divided to basal control, 8 weeks control, MICT, and HIIT groups. Exercise training groups complete 8 weeks and 5 days per weeks running on rodent’s treadmill. In MICT groups, the intensity and duration of training protocol was 50-75% of VO2max, and 15 to 60 minutes, respectively. In HIIT group, rats run 4-8×1 min with 70-100% of VO2 max and 1 min active recovery with 50-70% VO2max. Adipose tissue samples were taken 48 hours after last training session. FGF2 gene expression in adipose tissues was assessed with Real-Time PCR methods.
Results: Results showed that in the both of experimental groups, FGF2 gene expression in subcutaneous tissue increased but not significantly (P≥0.05). In contrast to these results, in visceral adipose tissue, MICT and HIIT training caused to none significant decrease in mRNA of FGF2 gene (P≥0.05).
Conclusions: Affording to the results, it’s seems that there is different response to exercise training in visceral and subcutaneous adipose tissue. It is likely that these two tissues have different angiogenic activity, although there are several different angiogenic factors involved in this process, this requires more study.

Rojas-Rodriguez R, Gealekman O, Kruse ME, Rosenthal B, Rao K, Min S, et al. Adipose tissue angiogenesis assay. Method Enzymol. 2014; 537:75.
Christiaens V, Lijnen HR. Angiogenesis and development of adipose tissue. Mol Cell Endocrinol. 2010; 318(1):2-9.
Lijnen HR. Angiogenesis and obesity. Cardiovasc Res. 2007; 78 (2):286-293.
Zoya Tahergurabi, Khazaei M. Obesity and Angiogenesis. Journal of Isfahan Medical School. 2012; 29:173.
Korivi M, Hou C-W, Chen C-Y, Lee J-P, Kesireddy SR, Kuo C-H. Angiogenesis: Role of exercise training and aging. Adapt Med. 2010; 2:29-41.
Cao R, Brakenhielm E, Wahlestedt C, Thyberg J, Cao Y. Leptin induces vascular permeability and synergistically stimulates angiogenesis with FGF-2 and VEGF. P Natl Acad Sci USA. 2001; 98(11):6390-5.
Powers C, McLeskey S, Wellstein A. Fibroblast growth factors, their receptors and signaling. Endocr-Relat Cancer. 2000; 7:165-97.
Pang C, Gao Z, Yin J, Zhang J, Jia W, Ye J. Macrophage infiltration into adipose tissue may promote angiogenesis for adipose tissue remodeling in obesity. Am J Physiol-Endoc M. 2008; 295(2):E313-E22.
Cullberg KB, Christiansen T, Paulsen SrK, Bruun JM, Pedersen SBnk, Richelsen Br. Effect of weight loss and exercise on angiogenic factors in the circulation and in adipose tissue in obese subjects. Obesity. 2010; 21(3):454-60.
Disanzo BL, You T. Effects of exercise training on indicators of adipose tissue angiogenesis and hypoxia in obese rats. Metabolism. 2014; 63(4):452-5.
Hatano D, Ogasawara J, Endoh S, Sakurai T, Nomura S, Kizaki T, et al. Effect of exercise training on the density of endothelial cells in the white adipose tissue of rats. Scand J Med Sci Spor. 2011; 21(6):e115-e21.
Czarkowska-Paczek B, Zendzian-Piotrowska M, Bartlomiejczyk I, Przybylski J, Gorski J. The influence of physical exercise on the generation of TGF-β, PDGF-AA, and VEGF-A in adipose tissue. Eur J Appl Physiol. 2011; 111(5):875-81.
Ye J, Gao Z, Yin J, He Q. Hypoxia is a potential risk factor for chronic inflammation and adiponectin reduction in adipose tissue of ob/ob and dietary obese mice. Am J Physiol-Endoc M. 2007. 293 (4): E1118-E1128.
Ramos JS, Dalleck LC, Tjonna AE, Beetham KS, Coombes JS. The impact of high-intensity interval training versus moderate-intensity continuous training on vascular function: a systematic review and meta-analysis. Sports Med. 2015; 45(5):679-92.
Lee Y, Min K, Talbert EE, Kavazis AN, Smuder AJ, Willis WT, et al. Exercise protects cardiac mitochondria against ischemia-reperfusion injury. Med Sci Sports Exerc. 2012; 44(3):397-405.
Rahimi M, Shekarforoush S, Asgari AR, Khoshbaten A, Rajabi H, Bazgir B, et al. The effect of high intensity interval training on cardioprotection against ischemia-reperfusion injury in wistar rats. Excli journal. 2015; 14:237.
Gollisch KS, Brandauer J, Jessen N, Toyoda T, Nayer A, Hirshman MF, et al. Effects of exercise training on subcutaneous and visceral adipose tissue in normal-and high-fat diet-fed rats. Am J Physiol-Endoc M. 2009; 297(2):E495.
Pasarica M, Sereda OR, Redman LM, Albarado DC, Hymel DT, Roan LE, et al. Reduced adipose tissue oxygenation in human obesity. Diabetes. 2009; 58(3):718-25.
Fain JN, Madan AK, Hiler ML, Cheema P, Bahouth SW. Comparison of the release of adipokines by adipose tissue, adipose tissue matrix, and adipocytes from visceral and subcutaneous abdominal adipose tissues of obese humans. Endocrinology. 2004; 145(5):2273-82.
Walton RG, Finlin BS, Mula J, Long DE, Zhu B, Fry CS, et al. Insuline-resistant subjects have normal angiogenic response to aerobic exercise training in skeletal muscle, but not in adipose tissue.‎ Physiol Rep. 2015; 3(6):e12415.
Mehri Alvar. Y, Sayevand. Z, Erfani Adab. F, Heydari Moghadam. R, Samavat Sharif. M.A, Karami. S. The effects of five weeks’ resistance training on some vascular growth factors in sedentary men. Sport Physiology. Spring 2016; 8 (29): 15-30. (Persian)
Coker RH, Williams RH, Kortebein PM, Sullivan DH, Evans WJ. Influence of exercise intensity on abdominal fat and adiponectin in elderly adults. Metab Syndr Relat Disord. 2009; 7(4):363-8.
Ohkawara K, Tanaka S, Miyachi M, Ishikawa-Takata K, Tabata I. A dose–response relation between aerobic exercise and visceral fat reduction: systematic review of clinical trials. Int J Obesity. 2007; 31(12):1786-97.
ACSM Fea. Exercise for Persons with Cardiovascular Disease American College of Sports Medicine 2016 [Available from: https://www.acsm.org/docs/current-comments/exercise-for-persons-with-cardiovascular-disease.pdf?sfvrsn=6.
WHO, 2010. Global Recommendations on Physical Activity for Health. Cataloguing-in-Publication Data 2010.