اثر تمرین استقامتی بر بیان میر 196 و میر 133 در بافت چربی سفید زیر پوستی در موش های صحرایی نژاد ویستار

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشگاه آیت اله العظمی بروجردی (ره)- گروه تربیت بدنی

2 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران

3 گروه بیوشیمی، انستیتو پاستور ایران

چکیده

هدف: هدف از این پژوهش، بررسی اثر تمرین استقامتی بر بیان میر-196 و میر-133 (miR-196a , miR-133a) در بافت چربی سفید زیر پوستی است. روش شناسی: 16 موش صحرایی نژاد ویستار به صورت تصادفی به دو گروه؛ 1)کنترل (تعداد:هشت) و 2) تمرین استقامتی (تعداد:هشت) تقسیم شدند. موش­های گروه­های تمرینی، به مدت هشت هفته، تحت تمرین استقامتی تداومی بر روی نوارگردان قرار گرفتند. برای اندازه­گیری بیان نسبی ژن های هدف از روش ریل تایم پی سی آر (Real Time–PCR) استفاده شد. نتایج: داده­ها نشان داد که بیان نسبی ژن «پروتئین غیر جفت کننده یک» (UCP1) در گروه استقامتی در قیاس با گروه کنترل به صورت معناداری بیشتر بود (p <0/05). با این حال، بیان میر-196 و میر-133 (miR-196a , miR-133a) در گروه استقامتی در قیاس با گروه کنترل از نظر آماری تفاوت معناداری نداشت (P>0/05).نتیجه­گیری: یافته های این مطالعه نشان داد که تمرین استقامتی تغییری در بیان میر-196 و میر-133 (miR-196a , miR-133a) در بافت چربی سفید زیرپوستی ایجاد نمی کند؛ دلالت بر این که «بیش تنظیمی بیان ژن پروتئین غیر جفت کننده یک (UCP1) ناشی از تمرین استقامتی»، با تغییرات بیان میر-196 و میر-133 مرتبط نیست.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Endurance Training on miR-196a and miR-133a Expression in Subcutaneous White Adipose Tissue of Wistar Rats

نویسندگان [English]

  • Saeed Daneshyar 1
  • Mohammad Reza Kordi 2
  • Mehdi Kadivar 3
  • Samane Afshari 2
1 Department of Physical Education, University of Ayatollah Alozma Boroujerdi
2 Department of exercise physiology, Faculty of Physical Education and Sports Sciences,Tehran University
3 Department of Biochemistry, Pasteur Institute of Iran
چکیده [English]

Purpose: The aim of current study was to investigate the effect of endurance training on expression of miR-196a and miR-133a in subcutaneous white adipose tissue (WAT).
Methods: 16 wistar rats were divided into two groups included: 1) Control (n=8) and 2) Endurance Training (n=8). The subjects of training group underwent continues endurance training on treadmill for eight weeks. To measure of gene expression were used the Real Time (RT) –PCR method.
Results: Data showed that gene expression of UCP1 was significantly higher in trained group than control (P0.05).
Conclusions: The results of this study showed that the endurance training did not change the expression of miR-196a and miR-133a in subcutaneous WAT, indicating that the endurance training-induced the increase of UCP1 expression did not relate to change of miR-196a and miR-133a expression.

کلیدواژه‌ها [English]

  • endurance training
  • miR 196a
  • miR 133a
  • White adipose tissue

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سابقه مقاله

  • تاریخ دریافت: 24 اردیبهشت 1396
  • تاریخ بازنگری: 19 بهمن 1399
  • تاریخ پذیرش: 11 دی 1399
  • تاریخ اولین انتشار: 11 دی 1399
  • تاریخ انتشار: 01 اسفند 1397

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