نوع مقاله : علمی - پژوهشی
نویسندگان
1 دانشگاه آیت اله العظمی بروجردی (ره)- گروه تربیت بدنی
2 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، دانشگاه تهران
3 گروه بیوشیمی، انستیتو پاستور ایران
چکیده
هدف: هدف از این پژوهش، بررسی اثر تمرین استقامتی بر بیان میر-196 و میر-133 (miR-196a , miR-133a) در بافت چربی سفید زیر پوستی است. روش شناسی: 16 موش صحرایی نژاد ویستار به صورت تصادفی به دو گروه؛ 1)کنترل (تعداد:هشت) و 2) تمرین استقامتی (تعداد:هشت) تقسیم شدند. موشهای گروههای تمرینی، به مدت هشت هفته، تحت تمرین استقامتی تداومی بر روی نوارگردان قرار گرفتند. برای اندازهگیری بیان نسبی ژن های هدف از روش ریل تایم پی سی آر (Real Time–PCR) استفاده شد. نتایج: دادهها نشان داد که بیان نسبی ژن «پروتئین غیر جفت کننده یک» (UCP1) در گروه استقامتی در قیاس با گروه کنترل به صورت معناداری بیشتر بود (p <0/05). با این حال، بیان میر-196 و میر-133 (miR-196a , miR-133a) در گروه استقامتی در قیاس با گروه کنترل از نظر آماری تفاوت معناداری نداشت (P>0/05).نتیجهگیری: یافته های این مطالعه نشان داد که تمرین استقامتی تغییری در بیان میر-196 و میر-133 (miR-196a , miR-133a) در بافت چربی سفید زیرپوستی ایجاد نمی کند؛ دلالت بر این که «بیش تنظیمی بیان ژن پروتئین غیر جفت کننده یک (UCP1) ناشی از تمرین استقامتی»، با تغییرات بیان میر-196 و میر-133 مرتبط نیست.
کلیدواژه‌ها
عنوان مقاله [English]
Effect of Endurance Training on miR-196a and miR-133a Expression in Subcutaneous White Adipose Tissue of Wistar Rats
نویسندگان [English]
- Saeed Daneshyar 1
- Mohammad Reza Kordi 2
- Mehdi Kadivar 3
- Samane Afshari 2
1 Department of Physical Education, University of Ayatollah Alozma Boroujerdi
2 Department of exercise physiology, Faculty of Physical Education and Sports Sciences,Tehran University
3 Department of Biochemistry, Pasteur Institute of Iran
چکیده [English]
Purpose: The aim of current study was to investigate the effect of endurance training on expression of miR-196a and miR-133a in subcutaneous white adipose tissue (WAT).
Methods: 16 wistar rats were divided into two groups included: 1) Control (n=8) and 2) Endurance Training (n=8). The subjects of training group underwent continues endurance training on treadmill for eight weeks. To measure of gene expression were used the Real Time (RT) –PCR method.
Results: Data showed that gene expression of UCP1 was significantly higher in trained group than control (P0.05).
Conclusions: The results of this study showed that the endurance training did not change the expression of miR-196a and miR-133a in subcutaneous WAT, indicating that the endurance training-induced the increase of UCP1 expression did not relate to change of miR-196a and miR-133a expression.
کلیدواژه‌ها [English]
- endurance training
- miR 196a
- miR 133a
- White adipose tissue
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