تاثیر هشت هفته تزریق IGF-1 و تمرین مقاومتی بر بیان ژن هایCOX-2 و p53 کولورکتال موش های صحرایی ویستار نر

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشگاه فردوسی مشهد

2 دانشیار گروه فیزیولوژی ورزشی دانشکده تربیت بدنی و علوم ورزشی دانشگاه فردوسی مشهد، مشهد، ایران

چکیده

هدف: هدف از مطالعه حاضر بررسی اثر هشت هفته تزریق IGF-1 و تمرین مقاومتی بر بیان ژن هایCox-2 و p53 در بافت کولورکتال موش های صحرایی نر بود. روش شناسی: در مجموع 28 سر موش صحرایی نر نژاد ویستار به طور تصادفی به چهار گروه شامل: تزریق سالین (کنترل)، تمرین مقاومتی+ تزریق سالین (تمرین)، تمرین مقاومتی+تزریق IGF-1 (ترکیبی)، تزریق IGF-1 (تزریق) تقسیم شدند. پروتکل تمرین مقاومتی (سه روز در هفته، 5 تکرار در سه دوره) شامل هشت هفته بالا رفتن از نرده با حمل باری که به دم بسته می شد. IGF-1 و سالین (5/1 میکروگرم در هر کیلوگرم در روز) قبل و پس از جلسات ورزشی تزریق می شد. سه روز پس از آخرین جلسه فعالیت ورزشی، بافت های کولورکتال جمع آوری شد و سطوح بیان ژن ها با استفاده از روش PCR ریل تایم اندازه گیری شد. نتایج: در گروه های تمرین، تزریق و ترکیبی، بیان ژن p53 در بافت کلورکتال نسبت به گروه کنترل کاهش یافته بود (05/0> p). کاهش معناداری در سطوح بیان ژن Cox-2 در گروه تمرین نسبت به گروه کنترل مشاهده شد (002/0= p). سطوح Cox-2 بطور معناداری در گروه تزریق از گروه کنترل بالاتر بود (002/0= p). در گروه ترکیبی، سطوح Cox-2 بطور معناداری بالاتر از گروه تمرین بود  (018/0= p). نتیجه گیری: در مجموع، این مطالعه نشان داد که تزریق IGF-1 سطوح p53 را کاهش و سطوح Cox-2 را در کولورکتال موش ها افزایش می دهد. با این وجود، تمرین مقاومتی بیان p53 و Cox-2 را کاهش می دهد و موجب تعدیل کاهش p53 و افزایش Cox-2 ناشی از تزریق IGF-1 می شود.

عنوان مقاله [English]

The effect of 8 weeks IGF-1 injection and resistance training on Cox-2 and p53 expression in colorectal of male Wistar rats

نویسندگان [English]

  • babisan askari 1
  • nahid bijeh 2
  • amir rashidlamir 2
1 PhD student in Sport Physiology, Faculty of Physical Education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
2 Department of Sport Physiology, Faculty of Physical Education and Sport Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]

Abstract
Purpose: The aim of this study was to determine the effect of 8 weeks IGF-1 injection and resistance training on Cox-2 and p53 expression in colorectal of male Wistar rats. Methods: A total of 28 male Wistar rats were randomly divided into 4 groups; saline injected control (Control), resistance training+saline injected (Training), resistance training+IGF-1 injected (Combined) and IGF-1 injected (Injection). Resistance training protocol consisted of climbing (three days/week, 5 reps/3 sets) a ladder carrying a load suspended from the tail for 8 weeks. IGF-1 and saline (1.5 µg/kg/day) were injected before and after exercise sessions. Three days after the last bout of exercise, colorectal tissues were collected and gene expression levels were analyzed using real-time PCR. Results: In Training, Combined and Injection groups, p53 expression in the colorectal decreased compared with Control (p

کلیدواژه‌ها [English]

  • Key Words: Exercise training
  • colorectal cancer
  • Insulin
  • like growth factor
  • 1
  • Apoptosis
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