بررسی تأثیر 10 هفته تمرین هوازی کم شدت همراه با محدودیت جریان خون بر میزان پروتئین فاکتور نروتروفیک مشتق شده از مغز(BDNF) در عضلات سلئوس، EDL و عصب سیاتیک موشهای صحرایی نر سالمند

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشجوی دکتری فیزیولوژی ورزش دانشگاه شهید بهشتی

2 دانشیار دانشگاه شهید بهشتی

3 1- مرکز تحقیقات فیزیولوژی، پژوهشکده علوم فیزیولوژی پایه و بالینی ، دانشگاه علوم پزشکی کرمان، کرمان، ایران 3- گروه فیزیولوژی و فارماکولوژی، دانشکده پزشکی، دانشگاه علوم پزشکی کرمان، کرمان، ایران

4 1- مرکز تحقیقات فیزیولوژی، پژوهشکده علوم فیزیولوژی پایه و بالینی ، دانشگاه علوم پزشکی کرمان، کرمان، ایران 2- مرکز تحقیقات قلب و عروق، پژوهشکده علوم فیزیولوژی پایه و بالینی ، دانشگاه علوم پزشکی کرمان، کرمان، ایران

چکیده

چکیدههدف: هدف از مطالعه حاضر بررسی تأثیر 10 هفته تمرین هوازی کم شدت همراه با محدودیت جریان خون بر میزان پروتئین فاکتور نروتروفیک مشتق شده از مغز(BDNF) در عضلات سلئوس و EDL و همچنین عصب سیاتیک موشهای صحرایی نر سالمند بود.روش تحقیق: 60 موش صحرایی نر کهنسال(23-24 ماه) نژاد ویستار، وزن بین 355 تا 481 گرم بودند که به 6 گروه تصادفی، گروه جراحی(BFR)، گروه جراحی ورزش(BFR+Ex)، گروه شم( Sham)، گروه شم ورزش(Sham+Ex)، کنترل( Ctl) و گروه کنترل ورزش(Ex) تقسیم شدند. گروه ها بعد از 10 هفته تمرین سبک هوازی (15متر/دقیقه) به مدت 1 ساعت، 5 جلسه در هفته تمرین کردند و 48 ساعت بعد از آخرین جلسه تمرین، قربانی شدند. نمونه عضلات و عصب سیاتیک بلافاصله جدا در نیتروژن جامد گذاشته و در دمای 80- درجه حفظ شدند. نمونه ها پروتئینی توسط روش وسترن بلات مورد سنجش قرار گرفتند. در این مطالعه تجزیه و تحلیل داده های تحقیق با استفاده از نرم افزار آماری  SPSSورژن 18انجام شد. توزیع طبیعی داده ها به وسیله آزمون شاپیرو - ویلک مشخص شد. برای آزمون فرضیه های تحقیق از تحلیل واریانس یک سویه استفاده گردید. همچنین جهت تفاوت معنی داری بین گروه های مختلف از آزمون تعقیبی توکی استفاده شد. سطح معناداری 0.05 P≤ در نظر گرفته شد.نتایج: نتایج نشان داد که پروتئین BDNF در عضله EDL درگروه BFR+Ex تنها با گروه های Ctl و Sh کاهش معنی دار(0.05P˂) داشت. همچنین در عضله سلئوس گروه BFR+Ex با تمامی گروه ها کاهش معنی داری(با گروه های Ctl ، Sh و BFR 0.00 =p و Ex و Sh+Ex 0.03 p˂ ) داشت. علاوه بر این گرو ه های  Ex، Sh+Ex نیز نسبت به گروه Ctl و Sh (0.00= p) کاهش معنی داری داشتند. از طرفی در عصب سیاتیک گروه BFR+Ex نسبت به همه ی گروه ها افزایش معنی داری(0.00= p) را نشان داد.  نتیجه گیری: این مطالعه نشان داد که این نوع تمرینات می تواند بر میزان پروتئین BDNF عضلات تند و کند انقباض تأثیر گذاشته و موجب جریان برگشتی از عضله به عصب شود. همچنین افزایش پروتئین BDNF بعد از این تمرینات در عصب سیاتیک مشاهده شد. و نشان داد که BFR+Ex به عنوان یک روش تمرینی می تواند روی عصب و عضله تأثیر مثبت بگذارد.کلمات کلیدی: تمرین استقامتی-فاکتورهای نروتروفیک- کاهش جریان خون- دوران پیری 

عنوان مقاله [English]

Investigation the effect of low-intensity aerobic training for 10 weeks along with blood flow restriction on amount of protein BDNF in soleus and EDL muscles as well as the sciatic nerve in aged male rats

نویسنده [English]

  • Mohammad Ali Bahreini pour 1
1 Exercise physiology PHD. studendent
چکیده [English]

Abstract
The current study aims to investigat the effect of low-intensity aerobic training for 10 weeks along with blood flow restriction on amount of protein BDNF in soleus and EDL muscles as well as the sciatic nerve in aged male rats.
Methods: 60 aged -Wistar male rats (23-24 months) were chosen which their weigh were 355 to 481 grams. They were divided into 6 groups randomly, blood flow restriction (BFR), exercise with blood flow restriction (BFR + Ex), sham(Sh), sham exercise (Sham + Ex), control (Ctl) and control exercise (Ex). They were trained with a low aerobic exercise (15 m / min) for 1 hour, 5 days a week for 10 weeks then were sacrificed 48 hours after the last training session. The samples of muscle and the sciatic nerve were separated immediately and they were put in a solid nitrogen. and Then,they were preserved at -80 ° C. The protein samples were detected by Western Bloting method. The statistical analysis were conducted with SPSS software version 18. Data normal distribution determind by Shapiro – Wilk test. Comparisons were performed among different groups by one-way ANOVA and post hoc Tukey’s test. P values less than 0.05 were considered as statistically significant.
Results: The results have shown that BDNF protein in the EDL muscle for BFR + Ex group significantly decreases only with Ctl and Sh groups (P˂0.05). In addtion, in soleus muscle BFR + Ex group significantly decreased in all groups (with groups Ctl, Sh and BFR 0.00 = p and Ex and Sh + Ex p˂0.03). moeover, Ex, Sh + Ex groups in comparision with Ctl and Sh have decreased significantly (0.00 = p). On the other hand, the sciatic nerve of BFR + Ex group, in comparision with all groups, increased significant (0.00 = p).

Conclusion: This study has indicates that this type of exercise affects on the BDNF protein in slow and fastwitch muscles. Furthermore, BFR+Ex , as a training method, can influence on nerve and muscule positively.
key words:Endurance training-neurotrophic factors- reduced blood flow- aging

کلیدواژه‌ها [English]

  • endurance training
  • Neurotrophic Factors
  • reduced blood flow
  • aging
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