نوع مقاله : علمی - پژوهشی
نویسندگان
1 گروه علوم ورزشی، دانشکده علوم ورزشی، دانشگاه بیرجند، بیرجند ایران،
2 گروه علوم دامی، دانشکده کشاورزی، دانشگاه بیرجند، بیرجند، ایران
3 گروه علوم ورزشی، دانشکده علوم ورزشی، دانشگاه بیرجند، بیرجند ایران
چکیده
هدف: سیرتوئین -3 (SIRT3) یک استیلزدای میتوکندریایی است که از طریق استیلاسیون پروتئین اگزو گوانین گلیکوسیلاز-1 (OGG1)، سبب ترمیم آسیب های DNA میتوکندریایی و جلوگیری از آپوپتوزیس سلولی می گردد. هدف از مطالعه حاضر بررسی تاثیر شش هفته تمرین تداومی و تناوبی شدید همراه با مصرف رزوراترول، بر سطوح SIRT3 وOGG1 بافـت کبد موش های صحرایی نر ویستار بود.
روشها: در این پژوهش 48 موش صحرایی بطور تصادفی به شش گروه هشت تایی شامل گروه های کنترل، مکمل، تمرین تداومی، تمرین تداومی + مکمل، تمرین تناوبی، و گروه تمرین تناوبی + مکمل تقسیم شدند. تمرینات تداومی و تناوبی به مدت شش هفته، شش روز در هفته، به ترتیب با سرعت 27 و 54 متر در دقیقه بر روی نوارگردان مخصوص موش ها اجرا گردید. گروههای دریافت کننده رزوراترول روزانه50 میلیگرم به ازای هر کیلوگرم وزن بدن مکمل به صورت گاواژ دریافت نمودند. پس از مداخله، حیوانات بی هوش و نمونه کبدی آن ها جهت تجزیه و تحلیل و انجام آزمون آماری تحلیل واریانس یک طرفه و آزمون تعقیبی توکی مورد استفاده قرار گردید.
نتایج : پس از شش هفته سطوح OGGI کبدی گروه های تمرین تداومی (0001/0=p) و تمرین تداومی + مکمل (0001/0=p) نسبت به گروه کنترل به طور معنی دار بیشتر بود. همچنین سطوح SIRT3 کبدی گروه های مکمل (01/0 =p)، تمرین تناوبی (04/0 =p)، تمرین تناوبی + مکمل (04/0 =p)، تمرین تداومی (001/0=p)، و تمرین تداومی + مکمل (001/0=p) نسبت به گروه کنترل پس از 6 هفته به طور معنی داری افزایش یافت.
نتیجه گیری: در مقایسه با تمرین تناوبی شدید، اجرای تمرینـات تدوامی به همراه مصرف رزوراترول می تواند بهبودی بیشتری در عوامل موثر بر بقای سلولی و طول عمر، مانند: SIRT3 و OGG1 ایجاد کند.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of vigorous continuous and interval exercise training along with resveratrol on SIRT3 and OGG1 proteins in the liver tissue of male Wistar rats
نویسندگان [English]
- Mohammad Esmaeil Afzalpour 1
- Hadi Sarir 2
- Zoufa Zanjirian 3
- Mohsen Moammadnia Ahmadi 3
- ELHAM GHASEMI 3
1 Department of Sport Sciences, Faculty Sport Sciences, University of Birjand, Iran
2 Department of Animal Sciences, Facult of Agriculture, University of Birjand, Iran
3 Department of Sport Sciences, Faculty Sport Sciences, University of Birjand, Iran
چکیده [English]
Purpose: Sirtuins-3 (SIRT3) is a mitochondrial deacetylase and through acetylation of Oxoguanine Glycosylase 1(OGG1) protein caused repair mitochondrial DNA damage and prevents cell apoptosis. The purpose of this study was to evaluate the effect of vigorous continuous and interval exercise training along with resveratrol on SIRT3 and OGG1 proteins in the liver tissue of male Wistar rats.
Methods: In this research, forty-eight male Albino Wistar rats divided into six equal of eight groups including sedentary control, supplement, interval exercise, interval exercise + supplement, continuous training and continuous training+ supplement. Continuous and interval training performed for 6 weeks, six days per week at speed of 27 and 54 m/min respectively on a rat treadmill. Resveratrol users received daily 50 mg/kg of body weight supplements as gavage. After intervention, the animals were anesthetized and their liver samples were analysed using one-way ANOVA and Tukey's post hoc tests.
Results: After 6 weeks OGG1 level was significantly increased in continuous exercise (p=0.0001) and continuous training + supplement (p = 0.0001) groups relative to the control group. Also, liver tissue SIRT3 levels in supplementation group (p = 0.01), interval training group (p = 0.04), interval training + supplement group (p = 0.04), continuous exercise group (p = 0.001), and continuous training + supplement group (P = 0.001) increased than control group after six weeks.
Conclusion: Compare to the interval training, performing of continuous training along with resveratrol consumption can induce more improvement in cell survival and life-span factors such as SIRT3 and OGG1.
کلیدواژه‌ها [English]
- Resveratrol
- Sirtuins-3
- Oxoguanine Glycosylase -1
- Continuous and Intensity Interval training
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