تاثیر یک جلسه فعالیت ورزشی تناوبی هوازی بر سطوح سرمی اسپکسین در مردان جوان فعال

نوع مقاله : علمی - پژوهشی

نویسندگان

1 دانشگاه مازندران

2 دانشگاه پیام نور گنبد کاووس

3 دانشگاه آزاد چالوس

چکیده

هدف: اسپکسین پپتید جدیدی است که در تنظیم رفتار تغذیه، وزن، متابولیسم انرژی و برداشت اسیدهای چرب به درون سلول­های چربی نقش دارد. هدف از مطالعه حاضر، بررسی تأثیر یک جلسه فعالیت­ورزشی تناوبی هوازی بر سطوح­سرمی­اسپکسین بود. روش­: یازده مرد جوان ­فعال (سن 3.63±24.00 سال، شاخص­­توده­­بدنی 2.00±22.98 کیلوگرم­بر­مترمربع) در این مطالعه شرکت نمودند. فعالیت­ورزشی شامل 2 سری (10ثانیه×6) رکاب­زدن با حداکثر سرعت و 10 دقیقه استراحت فعال (رکاب­زدن با شدت %75-%65 حداکثرضربان­قلب) بود. نمونه­های­خونی در وضعیت­ناشتا، قبل (30 دقیقه بعداز صرف­صبحانه حدود 365 کیلوکالری)، بلافاصله، 15، 30 و 45 دقیقه بعد از فعالیت ورزشی جمع­آوری شد. از آنالیز واریانس با اندازه­گیری­های مکرر و آزمون­تعقیبی بونفرونی برای بررسی تغییرات متغیرها در بازه­های زمانی مختلف و از ضریب­همبستگی پیرسون برای بررسی ارتباط بین متغیرها استفاده شد. یافته­ها: باوجود تغییر سطوح ­برخی از متابولیت­ها نظیر گلوکز، انسولین، لاکتات، TG و LDL-C در این پژوهش، تغییر معنی­داری در سطوح­ سرمی ­اسپکسین در هیچ یک از بازه­های زمانی اندازه­گیری شده، مشاهده­نشد (0.05p>). در حالت ناشتا و بلافاصله پس از فعالیت ­ورزشی، ارتباط معنی­داری بین سطوح اسپکسین و سایر متابولیت­ها یافت نشد (0.05p>) اما در 30 دقیقه پس از صرف صبحانه، سطوح­اسپکسین همبستگی منفی و معنی­داری با TG (0.032=p و 0.645- =r) و در طول دوره ریکاوری همبستگی مثبت و معنی­داری با HDL-C (0.041=p و 0.357+ =r) و همبستگی منفی و معنی­داری با انسولین داشت (0.023=p و 0.672- =r). نتیجه­گیری: به ­نظر می­رسد که تعادل ­منفی ­انرژی ناشی از یک جلسه فعالیت­ورزشی تناوبی هوازی، احتمالاً برای القای تغییر سطوح­ سرمی ­اسپکسین کافی نبوده­است.

کلیدواژه‌ها


عنوان مقاله [English]

The Effect of Single session of Aerobic Interval Exercise on Serum Spexin Levels in Active Young Men

چکیده [English]

Abstract
Aim: Spexin is a novel peptide which plays a role in the regulation of feeding behavior, body weight, energy metabolism, and long-chain fatty acid uptake into adipocytes. The aim of the current study was investigate the effect of single session of aerobic interval exercise on serum Spexin levels. Methods: Eleven active young men (aged 24±3.63 years, BMI 22.98±2 kg/m2) volunteered for this study. Acute exercise protocol comprising 2 series of 6×10 s sprinting cycling with maximal effort and 10 min active rest (cycling with intensity 65%–75% HRmax). Blood samples were obtained at fasting state, pre-exercise (30 min after breakfast ~365 kcal), immediately after, and 15, 30, and 45 min after exercise. The repeated measures of ANOVA and Bonferroni post-hoc test used to evaluate changes of parameters in the different times. The relationship between variables was assessed using the Pearson correlation coefficient. Results: Despite the changing levels of some metabolites such as glucose, insulin, lactate, TG and LDL-C in this study, spexin levels did not change significantly in any time courses of this study (p>0.05). At fasting state and post-exercise, spexin levels not correlated with other metabolites (p>0.05) but in 30 minutes after breakfast (pre-exercise) negatively correlated with TG (r= -0.645, p=0.032) and during the recovery period, positively correlated with HDL-C (r= +0.357, p=0.041) and negatively correlated with insulin (r= -0.672, p=0.023). Conclusion: It seems that acute exercise-induced negative energy balance in this study may not be sufficient to induce changes in Spexin serum levels.

کلیدواژه‌ها [English]

  • Spexin
  • Aerobic Interval Exercise
  • Active Young Men
- Beaulieu, K., Hopkins, M., Blundell, J., & Finlayson, G. (2016). Does habitual physical activity increase the sensitivity of the appetite control system? A systematic review. Sports Medicine, 22: 1-23.
- Blundell, J. E., Gibbons, C., Caudwell, P., Finlayson, G., & Hopkins, M. (2015). Appetite control and energy balance: impact of exercise. obesity reviews, 16(1): 67-76.
- Deighton, K., Karra, E., Batterham, R. L., & Stensel, D. J. (2013). Appetite, energy intake, and PYY3–36 responses to energy-matched continuous exercise and submaximal high-intensity exercise. Applied Physiology, Nutrition, and Metabolism, 38(9): 947-952.
- Dill, D. B., & Costill, D. L. (1974). Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. Journal of applied physiology, 37(2): 247-248.
- Flanagan, D. E., Evans, M. L., Monsod, T. P., Rife, F., Heptulla, R. A., Tamborlane, W. V., & Sherwin, R. S. (2003). The influence of insulin on circulating ghrelin. American Journal of Physiology-Endocrinology And Metabolism, 284(2): 313-316.
- Gu, L., Ma, Y., Gu, M., Zhang, Y., Yan, S., Li, N., ... & Peng, Y. (2015). Spexin peptide is expressed in human endocrine and epithelial tissues and reduced after glucose load in type 2 diabetes. Peptides, 71: 232-239.
- Hazell, T. J., Islam, H., Townsend, L. K., Schmale, M. S., & Copeland, J. L. (2016). Effects of exercise intensity on plasma concentrations of appetite-regulating hormones: Potential mechanisms. Appetite, 98: 80-88.
- Howe, S. M., Hand, T. M., & Manore, M. M. (2014). Exercise-trained men and women: role of exercise and diet on appetite and energy intake. Nutrients, 6(11): 4935-4960.
- Jahan-mihan, A., Magyari, P., Pinkstaff, S., Palamidy, V., Drake, K., & Quinn, C. (2016). The Effect of Intensity of Exercise on Appetite and Food Intake in Post-Exercise Period. The FASEB Journal, 30(1): 1161-8.
- Kumar, S., Hossain, J., Nader, N., Aguirre, R., Sriram, S., & Balagopal, P. B. (2016). Decreased Circulating Levels of Spexin in Obese Children. The Journal of Clinical Endocrinology & Metabolism, 101:2931-2936.
- Lam, C. K., Chari, M., Wang, P. Y., & Lam, T. K. (2008). Central lactate metabolism regulates food intake. American Journal of Physiology-Endocrinology and Metabolism, 295(2): 491-496.
- Ma, A., He, M. L., Bai, J., Ko, W. K., & Wong, A. O. L. (2016). Insulin As a Postprandial Signal for Spexin Induction in Fish Model: Signal Transduction and Evidence of a Peripheral Spexin Component. In Insulin Signaling/Insulin Action and Pathopathology of Diabetes (posters) (pp. SUN-734). Endocrine Society.
- Mazurek, K., Krawczyk, K., Zmijewski, P., Norkowski, H., & Czajkowska, A. (2014). Effects of aerobic interval training versus continuous moderate exercise programme on aerobic and anaerobic capacity, somatic features and blood lipid profile in collegate females. Annals of Agricultural and Environmental Medicine, 21(4): 844-849.
- Mirabeau, O., Severini, C., Audero, E., Gascuel, O., Possenti, R., Birney, E., ... & Gross, C. (2007). Identification of novel peptide hormones in the human proteome by hidden Markov model screening. Genome research, 17(3): 320-327.
- Mitchell, S., & Shaw, D. (2015). The worldwide epidemic of female obesity. Best Practice & Research Clinical Obstetrics & Gynaecology, 29(3): 289-299.
- Mozaffarian, D., Hao, T., Rimm, E. B., Willett, W. C., & Hu, F. B. (2011). Changes in diet and lifestyle and long-term weight gain in women and men. New England Journal of Medicine, 364(25): 2392-2404.
- Nagase, H., Bray, G. A., & York, D. A. (1996). Effects of pyruvate and lactate on food intake in rat strains sensitive and resistant to dietary obesity. Physiology & behavior, 59(3): 555-560.
- Page, K. A., & Melrose, A. J. (2016). Brain, hormone and appetite responses to glucose versus fructose. Current Opinion in Behavioral Sciences, 9: 111-117.
- Pearce, J., & Witten, K. (2016). Geographies of obesity: environmental understandings of the obesity epidemic. Abingdon, UK and New York.
- Pliquett, R. U., Fuhrer, D., Falk, S., Zysset, S., von Cramon, D. Y., Stumvoll, M. (2006). The regulation of circulating ghrelin – With recent updates from cell-based assays. Hormone and Metabolic Research, 38(7): 442-446.
- Pontzer, H., Durazo-Arvizu, R., Dugas, L. R., Plange-Rhule, J., Bovet, P., Forrester, T. E., ... & Luke, A. (2016). Constrained total energy expenditure and metabolic adaptation to physical activity in adult humans. Current Biology, 26(3): 410-417.
- Rocha, J., Paxman, J., Dalton, C., Winter, E., & Broom, D. (2015). Effects of an acute bout of aerobic exercise on immediate and subsequent three-day food intake and energy expenditure in active and inactive pre-menopausal women taking oral contraceptives. Appetite, 89: 183-191.
- Schubert, M. M., Sabapathy, S., Leveritt, M., & Desbrow, B. (2014). Acute exercise and hormones related to appetite regulation: a meta-analysis. Sports Medicine, 44(3): 387-403.
- Schultes, B., Schmid, S. M., Wilms, B., Jauch-Chara, K., Oltmanns, K. M., & Hallschmid, M. (2012). Lactate infusion during euglycemia but not hypoglycemia reduces subsequent food intake in healthy men. Appetite, 58(3): 818-821.
- Sim, A. Y., Wallman, K. E., Fairchild, T. J., & Guelfi, K. J. (2014). High-intensity intermittent exercise attenuates ad-libitum energy intake. International Journal of Obesity (Lond), 38(3): 417-22.
- Spriet, L. L. (2014). New insights into the interaction of carbohydrate and fat metabolism during exercise. Sports medicine, 44(1): 87-96.
- Strasser, B. (2013). Physical activity in obesity and metabolic syndrome. Annals of the New York Academy of Sciences, 1281(1): 141-159.
- Tanaka, H., Monahan, K. D., & Seals, D. R. (2001). Age-predicted maximal heart rate revisited. Journal of the American College of Cardiology, 37(1): 153-156.
- Ueda, S. Y., Yoshikawa, T., Katsura, Y., Usui, T., & Fujimoto, S. (2009). Comparable effects of moderate intensity exercise on changes in anorectic gut hormone levels and energy intake to high intensity exercise. Journal of Endocrinology, 203(3): 357-364.
- Walewski, J. L., Ge, F., Gagner, M., Inabnet, W. B., Pomp, A., Branch, A. D., & Berk, P. D. (2010). Adipocyte accumulation of long-chain fatty acids in obesity is multifactorial, resulting from increased fatty acid uptake and decreased activity of genes involved in fat utilization. Obesity surgery, 20(1): 93-107.
- Walewski, J. L., Ge, F., Lobdell, H., Levin, N., Schwartz, G. J., Vasselli, J. R., ... & Berk, P. D. (2014). Spexin is a novel human peptide that reduces adipocyte uptake of long chain fatty acids and causes weight loss in rodents with diet‐induced obesity. Obesity, 22(7): 1643-1652.
- Wingfield, H. L., Smith-Ryan, A. E., Melvin, M. N., Roelofs, E. J., Trexler, E. T., Hackney, A. C., ... & Ryan, E. D. (2015). The acute effect of exercise modality and nutrition manipulations on post-exercise resting energy expenditure and respiratory exchange ratio in women: a randomized trial. Sports medicine-open, 1(1): 1-11.
- Wong, M. K., Sze, K. H., Chen, T., Cho, C. K., Law, H. C., Chu, I. K., & Wong, A. O. (2013). Goldfish spexin: solution structure and novel function as a satiety factor in feeding control. American Journal of Physiology-Endocrinology and Metabolism, 305(3): 348-366.
- Zwetsloot, K. A., John, C. S., Lawrence, M. M., Battista, R. A., & Shanely, R. A. (2014). High-intensity interval training induces a modest systemic inflammatory response in active, young men. J Inflamm Res, 7: 9-17.